BMRB Entry 25133

Name: HOXD13 Solution NMR Chemical Shift Coordinates
Published: 2022-05-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25133

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: HOXD13 Solution NMR Chemical Shift Coordinates PubMed: 25491407

Deposition date: 2014-08-06 Original release date: 2022-05-12

Authors: Turner, Matthew; Ames, James

Citation: Turner, Matthew; Zhang, Yonghong; Carlson, Hanqian; Stadler, H Scott; Ames, James. "Chemical shift assignments of mouse HOXD13 DNA binding domain bound to duplex DNA" Biomol. NMR Assign. 9, 267-270 (2015).

Assembly members:
HOXD13, polymer, 74 residues, Formula weight is not available

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):
HOXD13: GSHMLEGRKKRVPYTKLQLK ELENEYAINKFINKDKRRRI SAATNLSERQVTIWFQNRRV KDKKIVSKLKDTVS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	227
15N chemical shifts	64
1H chemical shifts	317

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HOXD13	1

Entities:

Entity 1, HOXD13 74 residues - Formula weight is not available

1

GLY

SER

HIS

MET

LEU

GLU

GLY

ARG

LYS

2

ARG

VAL

PRO

TYR

THR

LYS

LEU

GLN

LEU

LYS

3

GLU

LEU

GLU

ASN

GLU

TYR

ALA

ILE

ASN

LYS

4

PHE

ILE

ASN

LYS

ASP

LYS

ARG

ILE

5

SER

ALA

THR

ASN

LEU

SER

GLU

ARG

GLN

6

VAL

THR

ILE

TRP

PHE

GLN

ASN

ARG

VAL

7

LYS

ASP

LYS

ILE

VAL

SER

LYS

LEU

LYS

8

ASP

THR

VAL

SER

Samples:

sample_1: HOXD13, [U-15N], 0.4 mM; H20 90%; D20 10%

sample_2: HOXD13, [U-13C;U-15N], 0.4 mM; H20 90%; D20 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 307 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH TOCSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts