BMRB Entry 25168
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25168
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: flpp3sol BMRB submission PubMed: 26004443
Deposition date: 2014-08-20 Original release date: 2015-10-23
Authors: Zook, James; Mo, Gina; Craciunescu, Felicia; Sisco, Nick; Hansen, Debra; Baravati, Bobby; Van Horn, Wade; Cherry, Brian; Fromme, Petra
Citation: Zook, James; Mo, Gina; Craciunescu, Felicia; Sisco, Nick; Hansen, Debra; Baravati, Bobby; Cherry, Brian; Van Horn, Wade; Fromme, Petra. "NMR Structure of Francisella tularensis Virulence Determinant Reveals Structural Homology to Bet v1 Allergen Proteins" Structure 23, 1116-1122 (2015).
Assembly members:
flpp3Sol_2, polymer, 119 residues, 13265.504 Da.
Natural source: Common Name: Francisella tularensis Taxonomy ID: 177416 Superkingdom: Bacteria Kingdom: not available Genus/species: Francisella tularensis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSET
Entity Sequences (FASTA):
flpp3Sol_2: MDKGTQYKDGYYITTLNYNF
NTVYNATLQAIQNGQTFDYK
SNPYDISVNKNNGTDAEIVS
ASDSDSTDSLQVAMKKLPNN
ATRISIKYGSQGNSIRSSAL
IGIIEGNIRYANTHHHHHH
| Data type | Count |
| 13C chemical shifts | 421 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 646 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | flpp3Sol 2 | 1 |
Entities:
Entity 1, flpp3Sol 2 119 residues - 13265.504 Da.
| 1 | MET | ASP | LYS | GLY | THR | GLN | TYR | LYS | ASP | GLY | ||||
| 2 | TYR | TYR | ILE | THR | THR | LEU | ASN | TYR | ASN | PHE | ||||
| 3 | ASN | THR | VAL | TYR | ASN | ALA | THR | LEU | GLN | ALA | ||||
| 4 | ILE | GLN | ASN | GLY | GLN | THR | PHE | ASP | TYR | LYS | ||||
| 5 | SER | ASN | PRO | TYR | ASP | ILE | SER | VAL | ASN | LYS | ||||
| 6 | ASN | ASN | GLY | THR | ASP | ALA | GLU | ILE | VAL | SER | ||||
| 7 | ALA | SER | ASP | SER | ASP | SER | THR | ASP | SER | LEU | ||||
| 8 | GLN | VAL | ALA | MET | LYS | LYS | LEU | PRO | ASN | ASN | ||||
| 9 | ALA | THR | ARG | ILE | SER | ILE | LYS | TYR | GLY | SER | ||||
| 10 | GLN | GLY | ASN | SER | ILE | ARG | SER | SER | ALA | LEU | ||||
| 11 | ILE | GLY | ILE | ILE | GLU | GLY | ASN | ILE | ARG | TYR | ||||
| 12 | ALA | ASN | THR | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
N-flpp3sol: flpp3Sol_2, [U-99% 13C; U-99% 15N], 0.8 mM; H2O 95%; D2O 5%; Sodium Chloride 90 mM; Sodium Phosphate 20 mM
NC-flpp3sol: flpp3Sol_2, [U-99% 13C; U-99% 15N], 0.8 mM; H2O 95%; D2O 5%; Sodium Chloride 90 mM; Sodium Phosphate 20 mM
Sample: ionic strength: 0.110 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.150 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | N-flpp3sol | isotropic | Sample |
| 3D HNCO | NC-flpp3sol | isotropic | Sample |
| HNcaCO (HNcaCO) | NC-flpp3sol | isotropic | Sample |
| 3D HNCA | NC-flpp3sol | isotropic | Sample |
| 3D HNCACB | NC-flpp3sol | isotropic | Sample |
| 3D CBCA(CO)NH | NC-flpp3sol | isotropic | Sample |
| 3D 1H-15N NOESY | NC-flpp3sol | isotropic | Sample |
| CcoNH (C_coNH.relayed) | NC-flpp3sol | isotropic | Sample |
| 3D HCCH-TOCSY | NC-flpp3sol | isotropic | Sample |
| 3D H(CCO)NH | NC-flpp3sol | isotropic | Sample |
| 3D 1H-13C NOESY | NC-flpp3sol | isotropic | Sample |
Software:
CcpNmr_Analysis v2.4, CCPN - Spectrum analysis
nmrDraw vany, Delaglio - Spectrum display
nmrPipe vany, Delaglio - Spectrum processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 850 MHz
Related Database Links:
| PDB | |
| EMBL | CAG46049 CAJ79085 CAL09432 |
| GB | AAX78073 ABI82600 ABK90252 ABO46387 ABU61155 |
| REF | WP_003015055 WP_003018179 WP_003034620 WP_003037248 WP_012429332 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts