BMRB Entry 25170

Name: Assignment for last 50 residues from three different organisms
Published: 2022-05-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25170

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Assignment for last 50 residues from three different organisms PubMed: 25412900

Deposition date: 2014-08-22 Original release date: 2022-05-12

Authors: Mishra, Pushpa

Citation: Mishra, Pushpa; Rajagopal, Sudarsan; Sharma, Shobhona; Hosur, Ramakrishna. "The C-terminal domain of eukaryotic acidic ribosomal P2 proteins is intrinsically disordered with conserved structural propensities" Protein Pept. Lett. 22, 212-218 (2014).

Assembly members:
HUP2-CTER, polymer, 50 residues, Formula weight is not available
TgP2-CTER, polymer, 50 residues, Formula weight is not available
PfP2-CTER, polymer, 50 residues, Formula weight is not available

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: not available Genus/species: not available not available

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PGEX4T3

Entity Sequences (FASTA):
HUP2-CTER: XAXGAVAVSAAXGSAAXAAG SAXAAAEEKKXEKKEESEES DDDXGFGLFD
TgP2-CTER: LQXXXSGGVAAAAAXXXXAA DAGAGAAAKKEXXXKEEEEE EDDMXFSXFD
PfP2-CTER: KLXXIGGGVAAAXAGAAXVE TAEAKKEXXKEEKKEEEEEX EDDLGFSLFG

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	342
15N chemical shifts	124
1H chemical shifts	448

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HUP2-CTER	1
2	TgP2-CTER	2
3	PfP2-CTER	3

Entities:

Entity 1, HUP2-CTER 50 residues - Formula weight is not available

1	X	ALA	X	GLY	ALA	VAL	ALA	VAL	SER	ALA
2	ALA	X	GLY	SER	ALA	ALA	X	ALA	ALA	GLY
3	SER	ALA	X	ALA	ALA	ALA	GLU	GLU	LYS	LYS
4	X	GLU	LYS	LYS	GLU	GLU	SER	GLU	GLU	SER
5	ASP	ASP	ASP	X	GLY	PHE	GLY	LEU	PHE	ASP

Entity 2, TgP2-CTER 50 residues - Formula weight is not available

1	LEU	GLN	X	X	X	SER	GLY	GLY	VAL	ALA
2	ALA	ALA	ALA	ALA	X	X	X	X	ALA	ALA
3	ASP	ALA	GLY	ALA	GLY	ALA	ALA	ALA	LYS	LYS
4	GLU	X	X	X	LYS	GLU	GLU	GLU	GLU	GLU
5	GLU	ASP	ASP	MET	X	PHE	SER	X	PHE	ASP

Entity 3, PfP2-CTER 50 residues - Formula weight is not available

1	LYS	LEU	X	X	ILE	GLY	GLY	GLY	VAL	ALA
2	ALA	ALA	X	ALA	GLY	ALA	ALA	X	VAL	GLU
3	THR	ALA	GLU	ALA	LYS	LYS	GLU	X	X	LYS
4	GLU	GLU	LYS	LYS	GLU	GLU	GLU	GLU	GLU	X
5	GLU	ASP	ASP	LEU	GLY	PHE	SER	LEU	PHE	GLY

Samples:

sample_1: HUP2-CTER, [U-98% 13C; U-98% 15N], 1 mM; H2O 90%; D2O 10%; TRIS 20 mM

sample_2: TgP2-CTER, [U-98% 13C; U-98% 15N], 1 mM; H2O 90%; D2O 10%; TRIS 20 mM

sample_3: PfP2-CTER, [U-98% 13C; U-98% 15N], 1 mM; H2O 90%; D2O 10%; TRIS 20 mM

sample_conditions_1: ionic strength: 20 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 7.4; temperature: 298 K

sample_conditions_3: pH: 7.4

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
HNN	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	X	ALA	X	GLY	ALA	VAL	ALA	VAL	SER	ALA
2	ALA	X	GLY	SER	ALA	ALA	X	ALA	ALA	GLY
3	SER	ALA	X	ALA	ALA	ALA	GLU	GLU	LYS	LYS
4	X	GLU	LYS	LYS	GLU	GLU	SER	GLU	GLU	SER
5	ASP	ASP	ASP	X	GLY	PHE	GLY	LEU	PHE	ASP

1	LEU	GLN	X	X	X	SER	GLY	GLY	VAL	ALA
2	ALA	ALA	ALA	ALA	X	X	X	X	ALA	ALA
3	ASP	ALA	GLY	ALA	GLY	ALA	ALA	ALA	LYS	LYS
4	GLU	X	X	X	LYS	GLU	GLU	GLU	GLU	GLU
5	GLU	ASP	ASP	MET	X	PHE	SER	X	PHE	ASP

1	LYS	LEU	X	X	ILE	GLY	GLY	GLY	VAL	ALA
2	ALA	ALA	X	ALA	GLY	ALA	ALA	X	VAL	GLU
3	THR	ALA	GLU	ALA	LYS	LYS	GLU	X	X	LYS
4	GLU	GLU	LYS	LYS	GLU	GLU	GLU	GLU	GLU	X
5	GLU	ASP	ASP	LEU	GLY	PHE	SER	LEU	PHE	GLY

1	X	ALA	X	GLY	ALA	VAL	ALA	VAL	SER	ALA
2	ALA	X	GLY	SER	ALA	ALA	X	ALA	ALA	GLY
3	SER	ALA	X	ALA	ALA	ALA	GLU	GLU	LYS	LYS
4	X	GLU	LYS	LYS	GLU	GLU	SER	GLU	GLU	SER
5	ASP	ASP	ASP	X	GLY	PHE	GLY	LEU	PHE	ASP

1	LEU	GLN	X	X	X	SER	GLY	GLY	VAL	ALA
2	ALA	ALA	ALA	ALA	X	X	X	X	ALA	ALA
3	ASP	ALA	GLY	ALA	GLY	ALA	ALA	ALA	LYS	LYS
4	GLU	X	X	X	LYS	GLU	GLU	GLU	GLU	GLU
5	GLU	ASP	ASP	MET	X	PHE	SER	X	PHE	ASP

1	LYS	LEU	X	X	ILE	GLY	GLY	GLY	VAL	ALA
2	ALA	ALA	X	ALA	GLY	ALA	ALA	X	VAL	GLU
3	THR	ALA	GLU	ALA	LYS	LYS	GLU	X	X	LYS
4	GLU	GLU	LYS	LYS	GLU	GLU	GLU	GLU	GLU	X
5	GLU	ASP	ASP	LEU	GLY	PHE	SER	LEU	PHE	GLY

1	X	ALA	X	GLY	ALA	VAL	ALA	VAL	SER	ALA
2	ALA	X	GLY	SER	ALA	ALA	X	ALA	ALA	GLY
3	SER	ALA	X	ALA	ALA	ALA	GLU	GLU	LYS	LYS
4	X	GLU	LYS	LYS	GLU	GLU	SER	GLU	GLU	SER
5	ASP	ASP	ASP	X	GLY	PHE	GLY	LEU	PHE	ASP

1	LEU	GLN	X	X	X	SER	GLY	GLY	VAL	ALA
2	ALA	ALA	ALA	ALA	X	X	X	X	ALA	ALA
3	ASP	ALA	GLY	ALA	GLY	ALA	ALA	ALA	LYS	LYS
4	GLU	X	X	X	LYS	GLU	GLU	GLU	GLU	GLU
5	GLU	ASP	ASP	MET	X	PHE	SER	X	PHE	ASP

1	LYS	LEU	X	X	ILE	GLY	GLY	GLY	VAL	ALA
2	ALA	ALA	X	ALA	GLY	ALA	ALA	X	VAL	GLU
3	THR	ALA	GLU	ALA	LYS	LYS	GLU	X	X	LYS
4	GLU	GLU	LYS	LYS	GLU	GLU	GLU	GLU	GLU	X
5	GLU	ASP	ASP	LEU	GLY	PHE	SER	LEU	PHE	GLY