BMRB Entry 25229

Name: Solution Structure of the Human FAAP20 UBZ
Published: 2014-12-01

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PDB ID: 2muq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25229
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the Human FAAP20 UBZ

Deposition date:

2014-09-16

Original release date:

2014-12-01

Authors:

Wojtaszek, Jessica; Wang, Su; Zhou, Pei

Citation:

Citation: Wojtaszek, Jessica; Wang, Su; Kim, Hyungjin; Wu, Qinglin; D'Andrea, Alan; Zhou, Pei. "Ubiquitin recognition by FAAP20 expands the complex interface beyond the canonical UBZ domain" Nucleic Acids Res. 42, 13997-14005 (2014).
PubMed: 25414354

Assembly members:

Assembly members:
UBZ, polymer, 44 residues, 4853.517 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UBZ: SHMGAAALRSCPMCQKEFAP RLTQLDVDSHLAQCLAESTE DVTW

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	142
15N chemical shifts	40
1H chemical shifts	283

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human FAAP20 UBZ	1
2	ZINC ION	2

Entities:

Entity 1, Human FAAP20 UBZ 44 residues - 4853.517 Da.

Residues 1-3 represent non-native residues leftover after protease cleavage removal of an affinity tag; residues 4-44 represent the UBZ domain of human FAAP20 (140-180).

1

SER

HIS

MET

GLY

ALA

LEU

ARG

SER

2

CYS

PRO

MET

CYS

GLN

LYS

GLU

PHE

ALA

PRO

3

ARG

LEU

THR

GLN

LEU

ASP

VAL

ASP

SER

HIS

4

LEU

ALA

GLN

CYS

LEU

ALA

GLU

SER

THR

GLU

5

ASP

VAL

THR

TRP

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D sparse-sampled HNCO	sample_2	isotropic	sample_conditions_1
3D sparse-sampled HNCA	sample_2	isotropic	sample_conditions_1
3D sparse-sampled HNCOCA	sample_2	isotropic	sample_conditions_1
3D sparse-sampled HNCACB	sample_2	isotropic	sample_conditions_1
3D sparse-sampled HNCOCACB	sample_2	isotropic	sample_conditions_1
3D sparse-sampled HACANH	sample_2	isotropic	sample_conditions_1
3D sparse-sampled HACACONH	sample_2	isotropic	sample_conditions_1
4D sparse-sampled HCccoNH TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
4D sparse-sampled HCCH TOCSY	sample_3	isotropic	sample_conditions_1
4D sparse-sampled CHCH NOESY	sample_3	isotropic	sample_conditions_1
4D sparse-sampled CHNH NOESY	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 25229

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: