BMRB Entry 25327

Name: Bd0108
Published: 2014-12-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25327

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Bd0108

Deposition date:

2014-11-11

Original release date:

2014-12-17

Authors:

Prehna, Gerd

Citation:

Citation: Prehna, Gerd; Ramirez, Benjamin; Lovering, Andrew. "The Lifestyle Switch Protein Bd0108 of Bdellovibrio bacteriovorus Is an Intrinsically Disordered Protein" PLoS One 9, e115390-e115390 (2014).
PubMed: 25514156

Assembly members:

Assembly members:
Bd0108, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source: Common Name: d-proteobacteria Taxonomy ID: 959 Superkingdom: Bacteria Kingdom: not available Genus/species: Bdellovibrio bacteriovorus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET26b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bd0108: GSHMADENANRPVNPGEDPN EAFRSTPFEATTSALGDCRE CIAYRTGATTGKGSRRHDDT VSREIKGSSATPGGSEKAGT GRQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	271
1H chemical shifts	288
15N chemical shifts	69

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bd0108	1

Entities:

Entity 1, Bd0108 83 residues - Formula weight is not available

1

GLY

SER

HIS

MET

ALA

ASP

GLU

ASN

ALA

ASN

2

ARG

PRO

VAL

ASN

PRO

GLY

GLU

ASP

PRO

ASN

3

GLU

ALA

PHE

ARG

SER

THR

PRO

PHE

GLU

ALA

4

THR

SER

ALA

LEU

GLY

ASP

CYS

ARG

GLU

5

CYS

ILE

ALA

TYR

ARG

THR

GLY

ALA

THR

6

GLY

LYS

GLY

SER

ARG

HIS

ASP

THR

7

VAL

SER

ARG

GLU

ILE

LYS

GLY

SER

ALA

8

THR

PRO

GLY

SER

GLU

LYS

ALA

GLY

THR

9

GLY

ARG

GLN

Samples:

sample_1: Bd0108, [U-99% 13C; U-99% 15N], 1 mM; TRIS 20 mM; sodium chloride 100 mM; beta-mercaptoethanol 2 mM; H2O 95%; D2O 5%

sample_conditions: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 0.1 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions
3D HNCACB	sample_1	isotropic	sample_conditions
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions
3D HNCO	sample_1	isotropic	sample_conditions
3D HCACO	sample_1	isotropic	sample_conditions
3D HNHA	sample_1	isotropic	sample_conditions
3D H(CCO)NH	sample_1	isotropic	sample_conditions
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis, chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 800 MHz
Bruker Avance 900 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks