BMRB Entry 25383

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25383

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Title:
Backbone chemical shift assignment of human TRAP1-NTD (60-296)
Deposition date:
2014-12-08
Original release date:
2015-01-06
Authors:
Yang, Yunhuang; Ramelot, Theresa; Mao, Lei; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael
Citation:

Citation: Yang, Yunhuang; Ramelot, Theresa; Mao, Lei; Xiao, Rong; Everett, John; Montelione, Gaetano; Kennedy, Michael. "Backbone chemical shift assignment of human TRAP1-NTD (60-296)"  .

Assembly members:

Assembly members:
human_TRAP1-NTD, polymer, 247 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: HR3376E-15.1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human_TRAP1-NTD: MGHHHHHHSHSTQTAEDKEE PLHSIISSTESVQGSTSKHE FQAETKKLLDIVARSLYSEK EVFIRELISNASDALEKLRH KLVSDGQALPEMEIHLQTNA EKGTITIQDTGIGMTQEELV SNLGTIARSGSKAFLDALQN QAEASSKIIGQFGVGFYSAF MVADRVEVYSRSAAPGSLGY QWLSDGSGVFEIAEASGVRT GTKIIIHLKSDCKEFSSEAR VRDVVTKYSNFVSFPLYLNG RRMNTLQ

Data sets:
Data typeCount
1H chemical shifts158
13C chemical shifts472
15N chemical shifts158

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human TRAP1-NTD1

Entities:

Entity 1, human TRAP1-NTD 247 residues - Formula weight is not available

1   METGLYHISHISHISHISHISHISSERHIS
2   SERTHRGLNTHRALAGLUASPLYSGLUGLU
3   PROLEUHISSERILEILESERSERTHRGLU
4   SERVALGLNGLYSERTHRSERLYSHISGLU
5   PHEGLNALAGLUTHRLYSLYSLEULEUASP
6   ILEVALALAARGSERLEUTYRSERGLULYS
7   GLUVALPHEILEARGGLULEUILESERASN
8   ALASERASPALALEUGLULYSLEUARGHIS
9   LYSLEUVALSERASPGLYGLNALALEUPRO
10   GLUMETGLUILEHISLEUGLNTHRASNALA
11   GLULYSGLYTHRILETHRILEGLNASPTHR
12   GLYILEGLYMETTHRGLNGLUGLULEUVAL
13   SERASNLEUGLYTHRILEALAARGSERGLY
14   SERLYSALAPHELEUASPALALEUGLNASN
15   GLNALAGLUALASERSERLYSILEILEGLY
16   GLNPHEGLYVALGLYPHETYRSERALAPHE
17   METVALALAASPARGVALGLUVALTYRSER
18   ARGSERALAALAPROGLYSERLEUGLYTYR
19   GLNTRPLEUSERASPGLYSERGLYVALPHE
20   GLUILEALAGLUALASERGLYVALARGTHR
21   GLYTHRLYSILEILEILEHISLEULYSSER
22   ASPCYSLYSGLUPHESERSERGLUALAARG
23   VALARGASPVALVALTHRLYSTYRSERASN
24   PHEVALSERPHEPROLEUTYRLEUASNGLY
25   ARGARGMETASNTHRLEUGLN

Samples:

NC_sample: human TRAP1-NTD, [U-100% 13C; U-100% 15N], 0.87 mM; sodium chloride 50 mM; MgCl2 2 mM; NaPi 20 mM; DTT 1 mM; D2O 10%; H2O 90%

NCDILV: human TRAP1-NTD, [U-13C; U-15N; U-2H] ILV-Methylated, 0.53 mM; sodium chloride 50 mM; MgCl2 2 mM; NaPi 20 mM; DTT 1 mM; D2O 10%; H2O 90%

sample_conditions_1: temperature: 293 K; pH: 6.5; pressure: ambient atm; ionic strength: 0.2 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNCDILVisotropicsample_conditions_1
2D 1H-13C HSQCNCDILVisotropicsample_conditions_1
3D HNCACBNCDILVisotropicsample_conditions_1
3D HNCOCACBNCDILVisotropicsample_conditions_1
3D HNCONCDILVisotropicsample_conditions_1
3D HN(CA)CONCDILVisotropicsample_conditions_1
3D HNCANCDILVisotropicsample_conditions_1
3D HN(CO)CANCDILVisotropicsample_conditions_1
3D 1H-15N NOESYNC_sampleisotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 850 MHz

Related Database Links:

PDB
DBJ BAD93042 BAD96769 BAD96796 BAD96925 BAG61180
EMBL CAH92662
GB AAA87704 AAC02679 AAC24722 AAF15314 AAH01455
PIR A55877
REF NP_001126557 NP_001258978 NP_001267429 NP_057376 XP_003269265
SP Q12931
AlphaFold Q12931

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks