BMRB Entry 25386

Name: Chemical shift assignments of Zinc finger Domain of Methionine amino peptidase 1 from Homo sapiens
Published: 2015-02-25

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25386

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Chemical shift assignments of Zinc finger Domain of Methionine amino peptidase 1 from Homo sapiens PubMed: 25921012

Deposition date: 2014-12-10 Original release date: 2015-02-25

Authors: Rachineni, Kavitha; Arya, Tarun; singarapu, kiran kumar; Bharatam, Jagadeesh; Addlagatta, Anthony

Citation: Rachineni, Kavitha; Arya, Tarun; Singarapu, Kiran kumar; Addlagatta, Anthony; Bharatam, Jagadeesh. "Chemical shift assignments of zinc finger domain of methionine aminopeptidase 1 (MetAP1) from Homo sapiens" Biomol NMR Assign. 9, 351-353 (2015).

Assembly members:
zinc_finger_domain_of_METAP1, polymer, 104 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a(+)

Entity Sequences (FASTA):
zinc_finger_domain_of_METAP1: MGSSHHHHHHSSGLVPRGSH MMAAVETRVCETDGCSSEAK LQCPTCIKLGIQGSYFCSQE CFKGSWATHKLLHKKAKDEK AKREVSSWTVEGDINTDPWA GYRY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	267
15N chemical shifts	78
1H chemical shifts	426

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	zinc finger domain of METap 1	1
2	ZINC ION, 1	2
3	ZINC ION, 2	2

Entities:

Entity 1, zinc finger domain of METap 1 104 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

ALA

VAL

GLU

THR

ARG

VAL

CYS

4

GLU

THR

ASP

GLY

CYS

SER

GLU

ALA

LYS

5

LEU

GLN

CYS

PRO

THR

CYS

ILE

LYS

LEU

GLY

6

ILE

GLN

GLY

SER

TYR

PHE

CYS

SER

GLN

GLU

7

CYS

PHE

LYS

GLY

SER

TRP

ALA

THR

HIS

LYS

8

LEU

HIS

LYS

ALA

LYS

ASP

GLU

LYS

9

ALA

LYS

ARG

GLU

VAL

SER

TRP

THR

VAL

10

GLU

GLY

ASP

ILE

ASN

THR

ASP

PRO

TRP

ALA

11

GLY

TYR

ARG

TYR

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zinc finger domain of METAP1, [U-98% 13C; U-98% 15N], 300 uM; Tris 25 mM; H2O 90%; D2O, U-2H, 10%

sample_2: zinc finger domain of METAP1, [U-98% 15N], 300 uM; Tris 25 mM; H2O 90%; D2O, U-2H, 10%

sample_conditions_1: ionic strength: 25 mM; pH: 8.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - data analysis

SPARKY, Goddard - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts