BMRB Entry 25417

Name: NMR resonance assignments of CadC 1-159 from E.coli
Published: 2015-05-18

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25417

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: NMR resonance assignments of CadC 1-159 from E.coli PubMed: 25979249

Deposition date: 2015-01-07 Original release date: 2015-05-18

Authors: Schlundt, Andreas; Sattler, Michael; Heydenreich, Thomas

Citation: Schlundt, Andreas; Heydenreich, Thomas; Buchner, Sophie; Janowski, Robert; Niessing, Dierk; Sattler, Michael; Jung, Kirsten. "Structural and functional analysis of the signal transducing linker in the pH-responsive one component system CadC of Escherichia coli" J. Mol. Biol. 427, 2548-2561 (2015).

Assembly members:
CadC, polymer, 162 residues, 18160.6 Da.

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETTrx1a

Entity Sequences (FASTA):
CadC: GAMAQQPVVRVGEWLVTPSI NQISRNGRQLTLEPRLIDLL VFFAQHSGEVLSRDELIDNV WKRSIVTNHVVTQSISELRK SLKDNDEDSPVYIATVPKRG YKLMVPVIWYSEEEGEEIML SSPPPIPEAVPATDSPSHSL NIQNTATPPEQSPVKSKRFT TF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	482
15N chemical shifts	156
1H chemical shifts	755

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CadC1-159	1

Entities:

Entity 1, CadC1-159 162 residues - 18160.6 Da.

Sequence starts with -2. GAM is cloning remnant, an additional A is part of the restriction site. The original sequence number starts with Q2.

1

GLY

ALA

MET

ALA

GLN

PRO

VAL

ARG

2

VAL

GLY

GLU

TRP

LEU

VAL

THR

PRO

SER

ILE

3

ASN

GLN

ILE

SER

ARG

ASN

GLY

ARG

GLN

LEU

4

THR

LEU

GLU

PRO

ARG

LEU

ILE

ASP

LEU

5

VAL

PHE

ALA

GLN

HIS

SER

GLY

GLU

VAL

6

LEU

SER

ARG

ASP

GLU

LEU

ILE

ASP

ASN

VAL

7

TRP

LYS

ARG

SER

ILE

VAL

THR

ASN

HIS

VAL

8

VAL

THR

GLN

SER

ILE

SER

GLU

LEU

ARG

LYS

9

SER

LEU

LYS

ASP

ASN

ASP

GLU

ASP

SER

PRO

10

VAL

TYR

ILE

ALA

THR

VAL

PRO

LYS

ARG

GLY

11

TYR

LYS

LEU

MET

VAL

PRO

VAL

ILE

TRP

TYR

12

SER

GLU

GLY

GLU

ILE

MET

LEU

13

SER

PRO

ILE

PRO

GLU

ALA

VAL

14

PRO

ALA

THR

ASP

SER

PRO

SER

HIS

SER

LEU

15

ASN

ILE

GLN

ASN

THR

ALA

THR

PRO

GLU

16

GLN

SER

PRO

VAL

LYS

SER

LYS

ARG

PHE

THR

17

THR

PHE

Samples:

sample_1: CadC, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%; BisTris"natural, abundance", 20 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1

Software:

CCPNMR_Analysis v2.3, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

UNP	P23890
AlphaFold	Q2M6H0

Biological Magnetic Resonance Data Bank