BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25555

Title: Structure of PvHCt, an antimicrobial peptide from shrimp litopenaeus vannamei

Deposition date: 2015-03-27 Original release date: 2015-05-26

Authors: Petit, Vanessa; Rolland, Jean-Luc; Blond, Alain; Djediat, Chakib; Peduzzi, Jean; Goulard, Christophe; Bachere, Evelyne; Dupont, Joelle; Destoumieux-Garzon, Delphine; Rebuffat, Sylvie

Citation: Petit, Vanessa; Rolland, Jean-Luc; Blond, Alain; Djediat, Chakib; Peduzzi, Jean; Goulard, Christophe; Bachere, Evelyne; Dupont, Joelle; Destoumieux-Garzon, Delphine; Rebuffat, Sylvie. "THE HEMOCYANIN-DERIVED PVHCT IS AN ALPHA-HELICAL ANTIFUNGAL PEPTIDE THAT SPECIFICALLY PERMEABILIZES FUNGAL MEMBRANES"  J. Biol. Chem. ., .-..

Assembly members:
entity, polymer, 23 residues, 2758.086 Da.

Natural source:   Common Name: white shrimp   Taxonomy ID: 6689   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Litopenaeus vannamei

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: FEDLPNFGHIQVKVFNHGEH IHH

Data sets:
Data typeCount
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 23 residues - 2758.086 Da.

1   PHEGLUASPLEUPROASNPHEGLYHISILE
2   GLNVALLYSVALPHEASNHISGLYGLUHIS
3   ILEHISHIS

Samples:

sample_1: entity 3.5 mM; DPC, [U-99% 2H], 350 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr v3.1, Bruker Biospin - chemical shift assignment, processing

TOPSPIN v3.2, Bruker Biospin - chemical shift assignment, processing

AURELIA v3.8, BRUKER - peak picking

X-PLOR v3.851, Brunger - refinement

NMR spectrometers:

  • Bruker Avance 400 MHz

Related Database Links:

BMRB 25631
PDB
GB ABR14693 AHY86471 AHY86472 AHY86474 AHY86475