BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25599

Title: Solution structure of Sds3 in complex with Sin3A   PubMed: 26124119

Deposition date: 2015-05-08 Original release date: 2015-07-13

Authors: Clark, Michael; Radhakrishnan, Ishwar

Citation: Clark, Michael; Marcum, Ryan; Graveline, Richard; Chan, Clarence; Xie, Tao; Chen, Zhonglei; Ding, Yujia; Zhang, Yongbo; Mondragon, Alfonso; David, Gregory; Radhakrishnan, Ishwar. "Structural Insights into the Assembly of the Histone Deacetylase-Associated Sin3L/Rpd3L Corepressor Complex"  Proc. Natl. Acad. Sci. U.S.A. 112, E3669-E3678 (2015).

Assembly members:
Sds3SID, polymer, 27 residues, 3125.556 Da.
Sin3HID, polymer, 125 residues, 14698.959 Da.

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMCSG7

Entity Sequences (FASTA):
Sds3SID: SNAAQLNYLLTDEQIMEDLR TLNKLKS
Sin3HID: SNAEHIYRCEDERFELDVVL ETNLATIRVLEAIQKKLSRL SAEEQAKFRLDNTLGGTSEV IHRKALQRIYADKAADIIDG LRKNPSIAVPIVLKRLKMKE EEWREAQRGFNKVWREQNEK YYLKS

Data sets:
Data typeCount
13C chemical shifts631
15N chemical shifts154
1H chemical shifts1005

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sds3SID1
2Sin3HID2

Entities:

Entity 1, Sds3SID 27 residues - 3125.556 Da.

1   SERASNALAALAGLNLEUASNTYRLEULEU
2   THRASPGLUGLNILEMETGLUASPLEUARG
3   THRLEUASNLYSLEULYSSER

Entity 2, Sin3HID 125 residues - 14698.959 Da.

1   SERASNALAGLUHISILETYRARGCYSGLU
2   ASPGLUARGPHEGLULEUASPVALVALLEU
3   GLUTHRASNLEUALATHRILEARGVALLEU
4   GLUALAILEGLNLYSLYSLEUSERARGLEU
5   SERALAGLUGLUGLNALALYSPHEARGLEU
6   ASPASNTHRLEUGLYGLYTHRSERGLUVAL
7   ILEHISARGLYSALALEUGLNARGILETYR
8   ALAASPLYSALAALAASPILEILEASPGLY
9   LEUARGLYSASNPROSERILEALAVALPRO
10   ILEVALLEULYSARGLEULYSMETLYSGLU
11   GLUGLUTRPARGGLUALAGLNARGGLYPHE
12   ASNLYSVALTRPARGGLUGLNASNGLULYS
13   TYRTYRLEULYSSER

Samples:

sample_1: Sin3HID, [U-100% 13C; U-100% 15N], 0.5 – 1 mM; Sds3SID0.5 – 1 mM; H2O 90%; D2O 10%

sample_2: Sds3SID, [U-100% 13C; U-100% 15N], 0.5 – 1 mM; Sin3HID0.5 – 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.12 M; pH: 6.7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Agilent DD2 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts