BMRB Entry 25619

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25619

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Title:
Backbone 1H, 13C, and 15N chemical shift assignments for N-SasA, the N-terminal domain of SasA, in complex with the CI domain of KaiC from the Thermosynechococcus elongatus BP-1 cyanobacterial species
Deposition date:
2015-05-16
Original release date:
2015-07-14
Authors:
Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy
Citation:

Citation: Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy. "A Protein Fold Switch Joins the Circadian Oscillator to Clock Output in Cyanobacteria"  Science 349, 324-328 (2015).
PubMed: 26113641

Assembly members:

Assembly members:
FTeSasA16107P16AF, polymer, 108 residues, Formula weight is not available
FTeCI17247R41AK173AF, polymer, 247 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cyanobacteria   Taxonomy ID: 146786   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FTeSasA16107P16AF: DYKDDDDKALSLLLFVANRP GDEEETAAIQAHIQQLPSNF SFELKVVPIGEQPYLLEEYK LVATPALIKVRPEPRQTLAG RKLLQKVDYWWPRWQREVAL DYKDDDDK
FTeCI17247R41AK173AF: DYKDDDDKAEVKKIPTMIEG FDDISHGGLPQGATTLVSGT SGTGKTLFAVQFLYNGITIF NEPGIFVTFEESPQDIIKNA LSFGWNLQSLIDQGKLFILD ASPDPDGQEVAGDFDLSALI ERIQYAIRKYKATRVSIDSV TAVFQQYDAASVVRREIFRL AFRLAQLGVTTIMTTERVDE YGPVARFGVEEFVSDNVVIL RNVLEGERRRRTVEILKLRG TTHMKGEYPFTINNGINIFD YKDDDDK

Data sets:
Data typeCount
13C chemical shifts235
15N chemical shifts69
1H chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1N-SasA1
2CI2

Entities:

Entity 1, N-SasA 108 residues - Formula weight is not available

1   ASPTYRLYSASPASPASPASPLYSALALEU
2   SERLEULEULEUPHEVALALAASNARGPRO
3   GLYASPGLUGLUGLUTHRALAALAILEGLN
4   ALAHISILEGLNGLNLEUPROSERASNPHE
5   SERPHEGLULEULYSVALVALPROILEGLY
6   GLUGLNPROTYRLEULEUGLUGLUTYRLYS
7   LEUVALALATHRPROALALEUILELYSVAL
8   ARGPROGLUPROARGGLNTHRLEUALAGLY
9   ARGLYSLEULEUGLNLYSVALASPTYRTRP
10   TRPPROARGTRPGLNARGGLUVALALALEU
11   ASPTYRLYSASPASPASPASPLYS

Entity 2, CI 247 residues - Formula weight is not available

1   ASPTYRLYSASPASPASPASPLYSALAGLU
2   VALLYSLYSILEPROTHRMETILEGLUGLY
3   PHEASPASPILESERHISGLYGLYLEUPRO
4   GLNGLYALATHRTHRLEUVALSERGLYTHR
5   SERGLYTHRGLYLYSTHRLEUPHEALAVAL
6   GLNPHELEUTYRASNGLYILETHRILEPHE
7   ASNGLUPROGLYILEPHEVALTHRPHEGLU
8   GLUSERPROGLNASPILEILELYSASNALA
9   LEUSERPHEGLYTRPASNLEUGLNSERLEU
10   ILEASPGLNGLYLYSLEUPHEILELEUASP
11   ALASERPROASPPROASPGLYGLNGLUVAL
12   ALAGLYASPPHEASPLEUSERALALEUILE
13   GLUARGILEGLNTYRALAILEARGLYSTYR
14   LYSALATHRARGVALSERILEASPSERVAL
15   THRALAVALPHEGLNGLNTYRASPALAALA
16   SERVALVALARGARGGLUILEPHEARGLEU
17   ALAPHEARGLEUALAGLNLEUGLYVALTHR
18   THRILEMETTHRTHRGLUARGVALASPGLU
19   TYRGLYPROVALALAARGPHEGLYVALGLU
20   GLUPHEVALSERASPASNVALVALILELEU
21   ARGASNVALLEUGLUGLYGLUARGARGARG
22   ARGTHRVALGLUILELEULYSLEUARGGLY
23   THRTHRHISMETLYSGLYGLUTYRPROPHE
24   THRILEASNASNGLYILEASNILEPHEASP
25   TYRLYSASPASPASPASPLYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks