BMRB Entry 25625

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25625

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone 1H, 13C, and 15N chemical shift assignments for the double mutant G89A,D91R of KaiB in complex with the CI domain of KaiC from the Thermosynechococcus elongatus BP-1 cyanobacterial species
Deposition date:
2015-05-16
Original release date:
2015-07-14
Authors:
Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy
Citation:

Citation: Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy. "A Protein Fold Switch Joins the Circadian Oscillator to Clock Output in Cyanobacteria"  Science 349, 324-328 (2015).
PubMed: 26113641

Assembly members:

Assembly members:
FTeKaiBGDF, polymer, 124 residues, Formula weight is not available
FTeCI17247R41AK173AF, polymer, 247 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cyanobacteria   Taxonomy ID: 146786   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermosynechococcus elongatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FTeKaiBGDF: DYKDDDDKMAPLRKTYVLKL YVAGNTPNSVRALKTLNNIL EKEFKGVYALKVIDVLKNPQ LAEEDKILATPTLAKVLPPP VRRIIGDLSNREKVLIALRL LYEEIGDQAEDDLGLEDYKD DDDK
FTeCI17247R41AK173AF: DYKDDDDKAEVKKIPTMIEG FDDISHGGLPQGATTLVSGT SGTGKTLFAVQFLYNGITIF NEPGIFVTFEESPQDIIKNA LSFGWNLQSLIDQGKLFILD ASPDPDGQEVAGDFDLSALI ERIQYAIRKYKATRVSIDSV TAVFQQYDAASVVRREIFRL AFRLAQLGVTTIMTTERVDE YGPVARFGVEEFVSDNVVIL RNVLEGERRRRTVEILKLRG TTHMKGEYPFTINNGINIFD YKDDDDK

Data sets:
Data typeCount
13C chemical shifts155
15N chemical shifts49
1H chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KaiB G89A D91R mutant, chian 11
2KaiB G89A D91R mutant, chain 21
3CI2

Entities:

Entity 1, KaiB G89A D91R mutant, chian 1 124 residues - Formula weight is not available

1   ASPTYRLYSASPASPASPASPLYSMETALA
2   PROLEUARGLYSTHRTYRVALLEULYSLEU
3   TYRVALALAGLYASNTHRPROASNSERVAL
4   ARGALALEULYSTHRLEUASNASNILELEU
5   GLULYSGLUPHELYSGLYVALTYRALALEU
6   LYSVALILEASPVALLEULYSASNPROGLN
7   LEUALAGLUGLUASPLYSILELEUALATHR
8   PROTHRLEUALALYSVALLEUPROPROPRO
9   VALARGARGILEILEGLYASPLEUSERASN
10   ARGGLULYSVALLEUILEALALEUARGLEU
11   LEUTYRGLUGLUILEGLYASPGLNALAGLU
12   ASPASPLEUGLYLEUGLUASPTYRLYSASP
13   ASPASPASPLYS

Entity 2, CI 247 residues - Formula weight is not available

1   ASPTYRLYSASPASPASPASPLYSALAGLU
2   VALLYSLYSILEPROTHRMETILEGLUGLY
3   PHEASPASPILESERHISGLYGLYLEUPRO
4   GLNGLYALATHRTHRLEUVALSERGLYTHR
5   SERGLYTHRGLYLYSTHRLEUPHEALAVAL
6   GLNPHELEUTYRASNGLYILETHRILEPHE
7   ASNGLUPROGLYILEPHEVALTHRPHEGLU
8   GLUSERPROGLNASPILEILELYSASNALA
9   LEUSERPHEGLYTRPASNLEUGLNSERLEU
10   ILEASPGLNGLYLYSLEUPHEILELEUASP
11   ALASERPROASPPROASPGLYGLNGLUVAL
12   ALAGLYASPPHEASPLEUSERALALEUILE
13   GLUARGILEGLNTYRALAILEARGLYSTYR
14   LYSALATHRARGVALSERILEASPSERVAL
15   THRALAVALPHEGLNGLNTYRASPALAALA
16   SERVALVALARGARGGLUILEPHEARGLEU
17   ALAPHEARGLEUALAGLNLEUGLYVALTHR
18   THRILEMETTHRTHRGLUARGVALASPGLU
19   TYRGLYPROVALALAARGPHEGLYVALGLU
20   GLUPHEVALSERASPASNVALVALILELEU
21   ARGASNVALLEUGLUGLYGLUARGARGARG
22   ARGTHRVALGLUILELEULYSLEUARGGLY
23   THRTHRHISMETLYSGLYGLUTYRPROPHE
24   THRILEASNASNGLYILEASNILEPHEASP
25   TYRLYSASPASPASPASPLYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks