BMRB Entry 25626

Name: Backbone 1H, 13C, and 15N chemical shift assignments for the double mutant G88A,D90R of KaiB from the Synechococcus elongatus PCC 7942 cyanobacterial species
Published: 2015-07-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25626

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N chemical shift assignments for the double mutant G88A,D90R of KaiB from the Synechococcus elongatus PCC 7942 cyanobacterial species

Deposition date:

2015-05-16

Original release date:

2015-07-14

Authors:

Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy

Citation:

Citation: Chang, Yonggang; Cohen, Susan; Phong, Connie; Myers, William; Kim, Yong-Ick; Tseng, Roger; Lin, Jenny; Zhang, Li; Boyd, Joseph; Lee, Yvonne; Kang, Shannon; Lee, David; Li, Sheng; Britt, R.; Rust, Michael; Golden, Susan; LiWang, Andy. "A Protein Fold Switch Joins the Circadian Oscillator to Clock Output in Cyanobacteria" Science 349, 324-328 (2015).
PubMed: 26113641

Assembly members:

Assembly members:
SeKaiBGDF, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source: Common Name: cyanobacteria Taxonomy ID: 32046 Superkingdom: Bacteria Kingdom: not available Genus/species: Synechococcus elongatus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SeKaiBGDF: MSPRKTYILKLYVAGNTPNS VRALKTLKNILEVEFQGVYA LKVIDVLKNPQLAEEDKILA TPTLAKVLPLPVRRIIGDLS DREKVLIALRLLYGELQDSD DFDYKDDDDK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	208
15N chemical shifts	66
1H chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KaiB G88A D90R mutant, chain 1	1
2	KaiB G88A D90R mutant, chain 2	1

Entities:

Entity 1, KaiB G88A D90R mutant, chain 1 110 residues - Formula weight is not available

1	MET	SER	PRO	ARG	LYS	THR	TYR	ILE	LEU	LYS
2	LEU	TYR	VAL	ALA	GLY	ASN	THR	PRO	ASN	SER
3	VAL	ARG	ALA	LEU	LYS	THR	LEU	LYS	ASN	ILE
4	LEU	GLU	VAL	GLU	PHE	GLN	GLY	VAL	TYR	ALA
5	LEU	LYS	VAL	ILE	ASP	VAL	LEU	LYS	ASN	PRO
6	GLN	LEU	ALA	GLU	GLU	ASP	LYS	ILE	LEU	ALA
7	THR	PRO	THR	LEU	ALA	LYS	VAL	LEU	PRO	LEU
8	PRO	VAL	ARG	ARG	ILE	ILE	GLY	ASP	LEU	SER
9	ASP	ARG	GLU	LYS	VAL	LEU	ILE	ALA	LEU	ARG
10	LEU	LEU	TYR	GLY	GLU	LEU	GLN	ASP	SER	ASP
11	ASP	PHE	ASP	TYR	LYS	ASP	ASP	ASP	ASP	LYS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 25626

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	MET	SER	PRO	ARG	LYS	THR	TYR	ILE	LEU	LYS
2	LEU	TYR	VAL	ALA	GLY	ASN	THR	PRO	ASN	SER
3	VAL	ARG	ALA	LEU	LYS	THR	LEU	LYS	ASN	ILE
4	LEU	GLU	VAL	GLU	PHE	GLN	GLY	VAL	TYR	ALA
5	LEU	LYS	VAL	ILE	ASP	VAL	LEU	LYS	ASN	PRO
6	GLN	LEU	ALA	GLU	GLU	ASP	LYS	ILE	LEU	ALA
7	THR	PRO	THR	LEU	ALA	LYS	VAL	LEU	PRO	LEU
8	PRO	VAL	ARG	ARG	ILE	ILE	GLY	ASP	LEU	SER
9	ASP	ARG	GLU	LYS	VAL	LEU	ILE	ALA	LEU	ARG
10	LEU	LEU	TYR	GLY	GLU	LEU	GLN	ASP	SER	ASP
11	ASP	PHE	ASP	TYR	LYS	ASP	ASP	ASP	ASP	LYS

1	MET	SER	PRO	ARG	LYS	THR	TYR	ILE	LEU	LYS
2	LEU	TYR	VAL	ALA	GLY	ASN	THR	PRO	ASN	SER
3	VAL	ARG	ALA	LEU	LYS	THR	LEU	LYS	ASN	ILE
4	LEU	GLU	VAL	GLU	PHE	GLN	GLY	VAL	TYR	ALA
5	LEU	LYS	VAL	ILE	ASP	VAL	LEU	LYS	ASN	PRO
6	GLN	LEU	ALA	GLU	GLU	ASP	LYS	ILE	LEU	ALA
7	THR	PRO	THR	LEU	ALA	LYS	VAL	LEU	PRO	LEU
8	PRO	VAL	ARG	ARG	ILE	ILE	GLY	ASP	LEU	SER
9	ASP	ARG	GLU	LYS	VAL	LEU	ILE	ALA	LEU	ARG
10	LEU	LEU	TYR	GLY	GLU	LEU	GLN	ASP	SER	ASP
11	ASP	PHE	ASP	TYR	LYS	ASP	ASP	ASP	ASP	LYS

1	MET	SER	PRO	ARG	LYS	THR	TYR	ILE	LEU	LYS
2	LEU	TYR	VAL	ALA	GLY	ASN	THR	PRO	ASN	SER
3	VAL	ARG	ALA	LEU	LYS	THR	LEU	LYS	ASN	ILE
4	LEU	GLU	VAL	GLU	PHE	GLN	GLY	VAL	TYR	ALA
5	LEU	LYS	VAL	ILE	ASP	VAL	LEU	LYS	ASN	PRO
6	GLN	LEU	ALA	GLU	GLU	ASP	LYS	ILE	LEU	ALA
7	THR	PRO	THR	LEU	ALA	LYS	VAL	LEU	PRO	LEU
8	PRO	VAL	ARG	ARG	ILE	ILE	GLY	ASP	LEU	SER
9	ASP	ARG	GLU	LYS	VAL	LEU	ILE	ALA	LEU	ARG
10	LEU	LEU	TYR	GLY	GLU	LEU	GLN	ASP	SER	ASP
11	ASP	PHE	ASP	TYR	LYS	ASP	ASP	ASP	ASP	LYS