BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25648

Title: Backbone chemical shift assignment of rat p75NTR transmembrane and juxtamembrane intracellular domains in lipid/protein nanodiscs   PubMed: 26287629

Deposition date: 2015-06-03 Original release date: 2015-09-16

Authors: Mineev, Konstantin; Goncharuk, Sergey; Arseniev, Alexander

Citation: Mineev, Konstantin; Goncharuk, Sergey; Kuzmichev, Pavel; Vilar, Marcal; Arseniev, Alexander. "NMR Dynamics of Transmembrane and Intracellular Domains of p75NTR in Lipid-Protein Nanodiscs"  Biophys. J. 109, 772-782 (2015).

Assembly members:
p75-TMDCD, polymer, 65 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEMEX-1

Entity Sequences (FASTA):
p75-TMDCD: MTRGTTDNLIPVYCSILAAV VVGLVAYIAFKRWNSCKQNK QGANSRPVNQTPPPEGEKLH SDSGI

Data sets:
Data typeCount
13C chemical shifts121
15N chemical shifts32
1H chemical shifts199

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p75-TMDCD1

Entities:

Entity 1, p75-TMDCD 65 residues - Formula weight is not available

Sequence numbering corresponds to the Uniprot ID P07174

1   METTHRARGGLYTHRTHRASPASNLEUILE
2   PROVALTYRCYSSERILELEUALAALAVAL
3   VALVALGLYLEUVALALATYRILEALAPHE
4   LYSARGTRPASNSERCYSLYSGLNASNLYS
5   GLNGLYALAASNSERARGPROVALASNGLN
6   THRPROPROPROGLUGLYGLULYSLEUHIS
7   SERASPSERGLYILE

Samples:

sample_1: p75-TMDCD, [U-99% 13C; U-99% 15N], 0.2 mM; MSP1 0.4 mM; DMPC 19.2 mM; DMPG 4.8 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 70 mM; pH: 6.0; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CARA v1.8, Keller and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P07174
BMRB 19673 25646 25647
DBJ BAC29775 BAG36408 BAG64358
EMBL CAA28783
GB AAB59544 AAD17943 AAH38365 AAH50309 AAI42256
PRF 1303336A
REF NP_001095948 NP_002498 NP_036742 NP_150086 XP_001090039
SP P07174 P08138 Q9Z0W1
TPG DAA18576
AlphaFold P07174 P08138 P07174 Q9Z0W1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts