BMRB Entry 25674

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25674

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone Assignment of the MALT1 Paracaspase by Solution NMR
Deposition date:
2015-06-25
Original release date:
2016-01-29
Authors:
Unnerstaale, Sofia; Nowakowski, Michal; Mayzel, Maxim; Orekhov, Vladislav; Agback, Tatiana
Citation:

Citation: Unnerstaale, Sofia; Nowakowski, Michal; Baraznenok, Vera; Stenberg, Gun; Lindberg, Jimmy; Mayzel, Maxim; Orekhov, Vladislav; Agback, Tatiana. "Backbone Assignment of the MALT1 Paracaspase by Solution NMR"  PLoS One 11, e0146496-e0146496 (2016).
PubMed: 26788853

Assembly members:

Assembly members:
MALT1(Casp-Ig3), polymer, 388 residues, 44130 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MALT1(Casp-Ig3): MLAKDKVALLIGNMNYREHP KLKAPLVDVYELTNLLRQLD FKVVSLLDLTEYEMRNAVDE FLLLLDKGVYGLLYYAGHGY ENFGNSFMVPVDAPNPYRSE NCLCVQNILKLMQEKETGLN VFLLDMCRKRNDYDDTIPIL DALKVTANIVFGYATCQGAE AFEIQHSGLANGIFMKFLKD RLLEDKKITVLLDEVAEDMG KCHLTKGKQALEIRSSLSEK RALTDPIQGTEYSAESLVRN LQWAKAHELPESMCLKFDCG VQIQLGFAAEFSNVMIIYTS IVYKPPEIIMCDAYVTDFPL DLDIDPKDANKGTPEETGSY LVSKDLPKHCLYTRLSSLQK LKEHLVFTVCLSYQYSGLED TVEDKQEVNVGKPLIAKLDM HRHHHHHH

Data sets:
Data typeCount
13C chemical shifts997
15N chemical shifts272
1H chemical shifts671

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MALT1 subunit 11

Entities:

Entity 1, MALT1 subunit 1 388 residues - 44130 Da.

1   METLEUALALYSASPLYSVALALALEULEU
2   ILEGLYASNMETASNTYRARGGLUHISPRO
3   LYSLEULYSALAPROLEUVALASPVALTYR
4   GLULEUTHRASNLEULEUARGGLNLEUASP
5   PHELYSVALVALSERLEULEUASPLEUTHR
6   GLUTYRGLUMETARGASNALAVALASPGLU
7   PHELEULEULEULEUASPLYSGLYVALTYR
8   GLYLEULEUTYRTYRALAGLYHISGLYTYR
9   GLUASNPHEGLYASNSERPHEMETVALPRO
10   VALASPALAPROASNPROTYRARGSERGLU
11   ASNCYSLEUCYSVALGLNASNILELEULYS
12   LEUMETGLNGLULYSGLUTHRGLYLEUASN
13   VALPHELEULEUASPMETCYSARGLYSARG
14   ASNASPTYRASPASPTHRILEPROILELEU
15   ASPALALEULYSVALTHRALAASNILEVAL
16   PHEGLYTYRALATHRCYSGLNGLYALAGLU
17   ALAPHEGLUILEGLNHISSERGLYLEUALA
18   ASNGLYILEPHEMETLYSPHELEULYSASP
19   ARGLEULEUGLUASPLYSLYSILETHRVAL
20   LEULEUASPGLUVALALAGLUASPMETGLY
21   LYSCYSHISLEUTHRLYSGLYLYSGLNALA
22   LEUGLUILEARGSERSERLEUSERGLULYS
23   ARGALALEUTHRASPPROILEGLNGLYTHR
24   GLUTYRSERALAGLUSERLEUVALARGASN
25   LEUGLNTRPALALYSALAHISGLULEUPRO
26   GLUSERMETCYSLEULYSPHEASPCYSGLY
27   VALGLNILEGLNLEUGLYPHEALAALAGLU
28   PHESERASNVALMETILEILETYRTHRSER
29   ILEVALTYRLYSPROPROGLUILEILEMET
30   CYSASPALATYRVALTHRASPPHEPROLEU
31   ASPLEUASPILEASPPROLYSASPALAASN
32   LYSGLYTHRPROGLUGLUTHRGLYSERTYR
33   LEUVALSERLYSASPLEUPROLYSHISCYS
34   LEUTYRTHRARGLEUSERSERLEUGLNLYS
35   LEULYSGLUHISLEUVALPHETHRVALCYS
36   LEUSERTYRGLNTYRSERGLYLEUGLUASP
37   THRVALGLUASPLYSGLNGLUVALASNVAL
38   GLYLYSPROLEUILEALALYSLEUASPMET
39   HISARGHISHISHISHISHISHIS

Samples:

sample_1: MALT1(Casp-Ig3), [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 mM; TRIS 10 mM; sodium chloride 50 mM; TCEP, [U-2H], 1 mM

sample_conditions_1: ionic strength: 60 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection, processing

CcpNmr_Analysis v2.2.2, CCPN - chemical shift assignment, data analysis, peak picking

TANCY, Isaksson and Orekhov - collection, peak picking, processing

SPARKY, Goddard - data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks