BMRB Entry 25676

Name: Backbone resonance assignments of the mutant NS4A N-terminal domain
Published: 2015-07-24

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25676

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments of the mutant NS4A N-terminal domain

Deposition date:

2015-06-30

Original release date:

2015-07-24

Authors:

Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Willbold, Dieter; Sklan, Ella; Koenig, Bernd

Citation:

Citation: Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Willbold, Dieter; Sklan, Ella; Koenig, Bernd. "Amino Terminal Region of Dengue Virus NS4A Cytosolic Domain Binds to Highly Curved Liposomes." Viruses 7, 4119-4130 (2015).
PubMed: 26197333

Assembly members:

Assembly members:
molecule_1, polymer, 48 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Aeropyrum pernix Taxonomy ID: 56636 Superkingdom: Archaea Kingdom: not available Genus/species: Aeropyrum pernix

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
molecule_1: SLTLNEITEEGRLPTFMTQK ARDALDNLAVLHTAEAGGRA YNHALSEL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	134
15N chemical shifts	41
1H chemical shifts	41

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NS4A mut	1

Entities:

Entity 1, NS4A mut 48 residues - Formula weight is not available

1

SER

LEU

THR

LEU

ASN

GLU

ILE

THR

GLU

2

GLY

ARG

LEU

PRO

THR

PHE

MET

THR

GLN

LYS

3

ALA

ARG

ASP

ALA

LEU

ASP

ASN

LEU

ALA

VAL

4

LEU

HIS

THR

ALA

GLU

ALA

GLY

ARG

ALA

5

TYR

ASN

HIS

ALA

LEU

SER

GLU

LEU

Samples:

NMR_sample: mutNS4A, [U-13C; U-15N], 200 uM; Sodium Phosphate 50 mM; Sodium Azide 0.03%; Sodium Chloride 150 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1.0 atm; temperature: 303.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	NMR_sample	isotropic	sample_conditions_1
3D HNCA	NMR_sample	isotropic	sample_conditions_1
3D HNCACO	NMR_sample	isotropic	sample_conditions_1
3D HNCACB	NMR_sample	isotropic	sample_conditions_1
3D HNCO	NMR_sample	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance III HD 700 MHz