BMRB Entry 25742
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25742
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Title: TBK1 recruitment to cytosol-invading Salmonella induces anti-bacterial autophagy PubMed: 27370208
Deposition date: 2015-08-03 Original release date: 2016-07-11
Authors: Thurston, T.; Allen, Mark; Ravenhill, B.; Karpiyevitch, M.; Bloor, S.; Kaul, A.; Matthews, S.; Komander, D.; Holden, D.; Bycroft, M.; Randow, F.; Matthews, A.
Citation: Thurston, Teresa Lm; Boyle, Keith; Allen, Mark; Ravenhill, Benjamin; Karpiyevich, Maryia; Bloor, Stuart; Kaul, Annie; Noad, Jessica; Foeglein, Agnes; Matthews, Sophie; Komander, David; Bycroft, Mark; Randow, Felix. "Recruitment of TBK1 to cytosol-invading Salmonella induces WIPI2-dependent antibacterial autophagy" EMBO J. 35, 1779-1792 (2016).
Assembly members:
OPTINEURIN, polymer, 32 residues, 3514.0982 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: HLTV plasmid
Entity Sequences (FASTA):
OPTINEURIN: GSRNIPIHSCPKCGEVLPDI
DTLQIHVMDCII
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 104 |
15N chemical shifts | 25 |
1H chemical shifts | 218 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | OPTINEURIN | 1 |
2 | ZINC ION | 2 |
Entities:
Entity 1, OPTINEURIN 32 residues - 3514.0982 Da.
1 | GLY | SER | ARG | ASN | ILE | PRO | ILE | HIS | SER | CYS | ||||
2 | PRO | LYS | CYS | GLY | GLU | VAL | LEU | PRO | ASP | ILE | ||||
3 | ASP | THR | LEU | GLN | ILE | HIS | VAL | MET | ASP | CYS | ||||
4 | ILE | ILE |
Entity 2, ZINC ION - 65.409 Da.
1 | ZN |
Samples:
sample_1: OPTINEURIN, [U-13C; U-15N], 1.5 mM; H2O 95%; D2O 5%; Tris 10 mM; NaCl 135 mM; B-mercaptoethanol 5 mM
sample_conditions_1: ionic strength: 150.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 293.000 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
NOESY | sample_1 | solution | sample_conditions_1 |
TOCSY | sample_1 | solution | sample_conditions_1 |
DQF-COSY | sample_1 | solution | sample_conditions_1 |
HSQC | sample_1 | solution | sample_conditions_1 |
HNCACB | sample_1 | solution | sample_conditions_1 |
CBCACONH | sample_1 | solution | sample_conditions_1 |
HNCO | sample_1 | solution | sample_conditions_1 |
HNCACO | sample_1 | solution | sample_conditions_1 |
HNHB | sample_1 | solution | sample_conditions_1 |
Software:
Ansig vany, Kraulis - chemical shift assignment
AutoDep v4.3, PDBe - collection
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts