BMRB Entry 25838
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25838
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the C-terminal intrinsically disordered domain of Alb3 PubMed: 26568381
Deposition date: 2015-10-06 Original release date: 2016-01-06
Authors: Sattler, Michael; Hennig, Janosch
Citation: Horn, Annemarie; Hennig, Janosch; Ahmed, Yasar; Stier, Gunter; Wild, Klemens; Sattler, Michael; Hennig, Janosch. "Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction" Nat. Commun. 6, 8875-8875 (2015).
Assembly members:
Alb3-A3CT, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d
Entity Sequences (FASTA):
Alb3-A3CT: MDENASKIISAGRAKRSIAQ
PDDAGERFRQLKEQEKRSKK
NKAVAKDTVELVEESQSESE
EGSDDEEEEAREGALASSTT
SKPLPEVGQRRSKRSKRKRT
V
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 153 |
15N chemical shifts | 79 |
1H chemical shifts | 119 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | A3CT monomer | 1 |
Entities:
Entity 1, A3CT monomer 101 residues - Formula weight is not available
1 | MET | ASP | GLU | ASN | ALA | SER | LYS | ILE | ILE | SER | ||||
2 | ALA | GLY | ARG | ALA | LYS | ARG | SER | ILE | ALA | GLN | ||||
3 | PRO | ASP | ASP | ALA | GLY | GLU | ARG | PHE | ARG | GLN | ||||
4 | LEU | LYS | GLU | GLN | GLU | LYS | ARG | SER | LYS | LYS | ||||
5 | ASN | LYS | ALA | VAL | ALA | LYS | ASP | THR | VAL | GLU | ||||
6 | LEU | VAL | GLU | GLU | SER | GLN | SER | GLU | SER | GLU | ||||
7 | GLU | GLY | SER | ASP | ASP | GLU | GLU | GLU | GLU | ALA | ||||
8 | ARG | GLU | GLY | ALA | LEU | ALA | SER | SER | THR | THR | ||||
9 | SER | LYS | PRO | LEU | PRO | GLU | VAL | GLY | GLN | ARG | ||||
10 | ARG | SER | LYS | ARG | SER | LYS | ARG | LYS | ARG | THR | ||||
11 | VAL |
Samples:
sample_1: Alb3-A3CT, [U-100% 13C; U-100% 15N], 0.45 mM; sodium phosphate 20 mM; sodium chloride 150 mM; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts