BMRB Entry 25925

Name: Chemical shift assignments of rv3053c from Mycobacterium tuberculosis in the oxidized state
Published: 2016-01-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25925

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments of rv3053c from Mycobacterium tuberculosis in the oxidized state

Deposition date:

2015-12-18

Original release date:

2016-01-12

Authors:

Feng, Yingang

Citation:

Citation: Liu, Ya-Jun; Song, Xiaxia; Li, Yifei; Xuan, Jinsong; Cui, Qiu; Wang, Jinfeng; Feng, Yingang. "Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin" FEBS Lett. 590, 387-395 (2016).
PubMed: 26786191

Assembly members:

Assembly members:
rv3053c, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mycobacterium tuberculosis Taxonomy ID: 1773 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
rv3053c: MTVTVYTKPACVQCSATSKA LDKQGIAYQKVDISLDSEAR DYVMALGYLQAPVVVAGNDH WSGFRPDRIKALAGAALTAL EHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	360
15N chemical shifts	88
1H chemical shifts	571

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rv3053c	1

Entities:

Entity 1, rv3053c 87 residues - Formula weight is not available

1

MET

THR

VAL

THR

VAL

TYR

THR

LYS

PRO

ALA

2

CYS

VAL

GLN

CYS

SER

ALA

THR

SER

LYS

ALA

3

LEU

ASP

LYS

GLN

GLY

ILE

ALA

TYR

GLN

LYS

4

VAL

ASP

ILE

SER

LEU

ASP

SER

GLU

ALA

ARG

5

ASP

TYR

VAL

MET

ALA

LEU

GLY

TYR

LEU

GLN

6

ALA

PRO

VAL

ALA

GLY

ASN

ASP

HIS

7

TRP

SER

GLY

PHE

ARG

PRO

ASP

ARG

ILE

LYS

8

ALA

LEU

ALA

GLY

ALA

LEU

THR

ALA

LEU

9

GLU

HIS

Samples:

sample_1: rv3053c, [U-13C; U-15N], 1.0 mM; potassium phosphate 50 mM; potassium chloride 100 mM; DSS 0.02 % w/v

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHANH	sample_1	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks