BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26040

Title: Solution structure of pseudin-2 analog (Ps-P)   PubMed: 28469145

Deposition date: 2016-04-18 Original release date: 2017-04-24

Authors: Jeon, Dasom; Kim, Jin-Kyoung; Shin, Areum; Kim, Yangmee

Citation: Jeon, Dasom; Jeong, Min-Cheol; Jacob, Binu; Bang, Jeong Kyu; Kim, Eun-Hee; Cheong, Chaejoon; Jung, In Duk; Park, Yoonkyung; Kim, Yangmee. "Investigation of cationicity and structure of pseudin-2 analogues for enhanced bacterial selectivity and anti-inflammatory activity"  Sci. Rep. 7, 1455-1455 (2017).

Assembly members:
Ps-P, polymer, 24 residues, 2730.264 Da.

Natural source:   Common Name: paradox frog   Taxonomy ID: 43558   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Pseudis paradoxa

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Ps-P: GLNALKKVFQPIHEAIKLIN NHVQ

Data sets:
Data typeCount
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 24 residues - 2730.264 Da.

1   GLYLEUASNALALEULYSLYSVALPHEGLN
2   PROILEHISGLUALAILELYSLEUILEASN
3   ASNHISVALGLN

Samples:

1mM_Ps-P: DPC 200 mM; H2O 90%; D2O 10%

200mM_DPC: pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESY1mM_Ps-Pisotropic200mM_DPC
2D 1H-1H TOCSY1mM_Ps-Pisotropic200mM_DPC
2D DQF-COSY1mM_Ps-Pisotropic200mM_DPC

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift calculation

NMR spectrometers:

  • Bruker Avance 700 MHz