BMRB Entry 26057
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR26057
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Title: Solution Structure of Mutant of BMAP-28(1-18)
Deposition date: 2016-05-12 Original release date: 2017-09-11
Authors: Agadi, Nutan; Kumar, Ashutosh
Citation: Agadi, Nutan; Kumar, Ashutosh. "Structure, Function And Membrane Interaction Studies of Two Synthetic Peptides Using Solution And Solid State NMR" .
Assembly members:
entity, polymer, 18 residues, 1999.514 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: IGLRGLGRKIALIHKKYG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 57 |
| 1H chemical shifts | 132 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 18 residues - 1999.514 Da.
| 1 | ILE | GLY | LEU | ARG | GLY | LEU | GLY | ARG | LYS | ILE | ||||
| 2 | ALA | LEU | ILE | HIS | LYS | LYS | TYR | GLY |
Samples:
sample_1: sodium phosphate 20 mM; D2O, [U-99% 2H], 10%; H2O 90%; SDS, [U-99% 2H], 50 mM
sample_conditions_1: ionic strength: 0 M; pH: 6.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - processing
ccpnmr, CCPN - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
NMR spectrometers:
- Bruker ASCEND 750 MHz
- Bruker Avance 700 MHz