BMRB Entry 26312

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26312

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Title:
Ufm1
Deposition date:
2021-08-15
Original release date:
2021-08-20
Authors:
Sasakawa, Hiroaki; Kato, Koichi
Citation:

Citation: Sasakawa, Hiroaki; Sakata, Eri; Yamaguchi, Yoshiki; Komatsu, Masaaki; Tatsumi, Kanako; Kominami, Eiki; Tanaka, Keiji; Kato, Koichi. "Solution structure and dynamics of Ufm1, a ubiquitin-fold modifier 1."  Biochem. Biophys. Res. Commun. 343, 21-26 (2006).
PubMed: 16527251

Assembly members:

Assembly members:
Ufm1, polymer, 90 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ufm1: GPLGSMSKVSFKITLTSDPR LPYKVLSVPESTPFTAVLKF AAEEFKVPAATSAIITNDGI GINPAQTAGNVFLKHGSELR IIPRDRVGSC

Data sets:
Data typeCount
13C chemical shifts235
15N chemical shifts81
1H chemical shifts463

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ufm11

Entities:

Entity 1, Ufm1 90 residues - Formula weight is not available

1   GLYPROLEUGLYSERMETSERLYSVALSER
2   PHELYSILETHRLEUTHRSERASPPROARG
3   LEUPROTYRLYSVALLEUSERVALPROGLU
4   SERTHRPROPHETHRALAVALLEULYSPHE
5   ALAALAGLUGLUPHELYSVALPROALAALA
6   THRSERALAILEILETHRASNASPGLYILE
7   GLYILEASNPROALAGLNTHRALAGLYASN
8   VALPHELEULYSHISGLYSERGLULEUARG
9   ILEILEPROARGASPARGVALGLYSERCYS

Samples:

sample_1: Ufm1, [U-13C; U-15N], 0.2 mM; sodium phosphate 10 mM; sodium chloride 100 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 100 mM; pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
15N-edited TOCSYsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

xwinnmr - processing

NMR spectrometers:

  • JEOL ECA 920 MHz
  • Bruker Avance 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

PDB
UNP P61960
AlphaFold Q9NZF2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks