BMRB Entry 26314

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26314

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Title:
Backbone 1H, 13C, 15N and Side chain CB Chemical Shift Assignments for Paip2A
Deposition date:
2021-09-07
Original release date:
2022-05-11
Authors:
Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori
Citation:

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Shunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1"  J. Biol. Chem. 298, 101844-101844 (2022).
PubMed: 35307347

Assembly members:

Assembly members:
Paip2A, polymer, 132 residues, 15396 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Paip2A: GPLGSMKDPSRSSTSPSIIN EDVIINGHSHEDDNPFAEYM WMENEEEFNRQIEEELWEEE FIERCFQEMLEEEEEHEWFI PARDLPQTMDQIQDQFNDLV ISDGSSLEDLVVKSNLNPNA KEFVPGVKYGNI

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts100
1H chemical shifts100

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 132 residues - 15396 Da.

1   GLYPROLEUGLYSERMETLYSASPPROSER
2   ARGSERSERTHRSERPROSERILEILEASN
3   GLUASPVALILEILEASNGLYHISSERHIS
4   GLUASPASPASNPROPHEALAGLUTYRMET
5   TRPMETGLUASNGLUGLUGLUPHEASNARG
6   GLNILEGLUGLUGLULEUTRPGLUGLUGLU
7   PHEILEGLUARGCYSPHEGLNGLUMETLEU
8   GLUGLUGLUGLUGLUHISGLUTRPPHEILE
9   PROALAARGASPLEUPROGLNTHRMETASP
10   GLNILEGLNASPGLNPHEASNASPLEUVAL
11   ILESERASPGLYSERSERLEUGLUASPLEU
12   VALVALLYSSERASNLEUASNPROASNALA
13   LYSGLUPHEVALPROGLYVALLYSTYRGLY
14   ASNILE

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks