BMRB Entry 26315
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26315
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Title: Backbone 1H, 15N and HA Chemical Shift Assignments for Paip2A(25-83) PubMed: 35307347
Deposition date: 2021-09-07 Original release date: 2022-05-11
Authors: Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori
Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Shunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1" J. Biol. Chem. 298, 101844-101844 (2022).
Assembly members:
Paip2A(25-83), polymer, 64 residues, 7995 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1
Entity Sequences (FASTA):
Paip2A(25-83): GPLGSHEDDNPFAEYMWMEN
EEEFNRQIEEELWEEEFIER
CFQEMLEEEEEHEWFIPARD
LPQT
- assigned_chemical_shifts
Data type | Count |
15N chemical shifts | 47 |
1H chemical shifts | 93 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 64 residues - 7995 Da.
1 | GLY | PRO | LEU | GLY | SER | HIS | GLU | ASP | ASP | ASN | ||||
2 | PRO | PHE | ALA | GLU | TYR | MET | TRP | MET | GLU | ASN | ||||
3 | GLU | GLU | GLU | PHE | ASN | ARG | GLN | ILE | GLU | GLU | ||||
4 | GLU | LEU | TRP | GLU | GLU | GLU | PHE | ILE | GLU | ARG | ||||
5 | CYS | PHE | GLN | GLU | MET | LEU | GLU | GLU | GLU | GLU | ||||
6 | GLU | HIS | GLU | TRP | PHE | ILE | PRO | ALA | ARG | ASP | ||||
7 | LEU | PRO | GLN | THR |
Samples:
sample_1: Paip2A(25-83), [U-100% 15N], 90 uM; potassium phosphate 18 mM; sodium chloride 90 mM; H2O 90%; D2O, [U-100% 2H], 10%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.190 - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts