BMRB Entry 26316

Name: Backbone 1H, 13C, 15N, HA and Side chain 13C Chemical Shift Assignments for RRM2 of PABPC1
Published: 2022-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26316

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, 15N, HA and Side chain 13C Chemical Shift Assignments for RRM2 of PABPC1 PubMed: 35307347

Deposition date: 2021-09-07 Original release date: 2022-05-11

Authors: Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Shunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1" J. Biol. Chem. 298, 101844-101844 (2022).

Assembly members:
RRM2 of PABPC1, polymer, 96 residues, 10768 Da.

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1

Entity Sequences (FASTA):
RRM2 of PABPC1: GPLGSNIFIKNLDKSIDNKA LYDTFSAFGNILSCKVVCDE NGSKGYGFVHFETQEAAERA IEKMNGMLLNDRKVFVGRFK SRKEREAELGARAKEF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	276
15N chemical shifts	93
1H chemical shifts	184

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 96 residues - 10768 Da.

1

GLY

PRO

LEU

GLY

SER

ASN

ILE

PHE

ILE

LYS

2

ASN

LEU

ASP

LYS

SER

ILE

ASP

ASN

LYS

ALA

3

LEU

TYR

ASP

THR

PHE

SER

ALA

PHE

GLY

ASN

4

ILE

LEU

SER

CYS

LYS

VAL

CYS

ASP

GLU

5

ASN

GLY

SER

LYS

GLY

TYR

GLY

PHE

VAL

HIS

6

PHE

GLU

THR

GLN

GLU

ALA

GLU

ARG

ALA

7

ILE

GLU

LYS

MET

ASN

GLY

MET

LEU

ASN

8

ASP

ARG

LYS

VAL

PHE

VAL

GLY

ARG

PHE

LYS

9

SER

ARG

LYS

GLU

ARG

GLU

ALA

GLU

LEU

GLY

10

ALA

ARG

ALA

LYS

GLU

PHE

Samples:

sample_1: RRM2 of PABPC1, [U-100% 13C; U-100% 15N], 455 uM; sodium phosphate 18 mM; sodium chloride 135 mM; H2O 50 M; D2O, [U-2H], 10%; DTT 0.9 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.190 - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts