BMRB Entry 26318

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26318

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Title:
Backbone 1H, 13C, 15N and CB Chemical Shift Assignments for RRM2/3
Deposition date:
2021-09-07
Original release date:
2022-05-11
Authors:
Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori
Citation:

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Shunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1"  J. Biol. Chem. 298, 101844-101844 (2022).
PubMed: 35307347

Assembly members:

Assembly members:
RRM2/3 of PABPC1, polymer, 195 residues, 22202 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM2/3 of PABPC1: GPLGSNIFIKNLDKSIDNKA LYDTFSAFGNILSCKVVCDE NGSKGYGFVHFETQEAAERA IEKMNGMLLNDRKVFVGRFK SRKEREAELGARAKEFTNVY IKNFGEDMDDERLKDLFGKF GPALSVKVMTDESGKSKGFG FVSFERHEDAQKAVDEMNGK ELNGKQIYVGRAQKKVERQT ELKRKFEQMKQDRIT

Data sets:
Data typeCount
13C chemical shifts314
15N chemical shifts182
1H chemical shifts182

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11

Entities:

Entity 1, subunit 1 195 residues - 22202 Da.

1   GLYPROLEUGLYSERASNILEPHEILELYS
2   ASNLEUASPLYSSERILEASPASNLYSALA
3   LEUTYRASPTHRPHESERALAPHEGLYASN
4   ILELEUSERCYSLYSVALVALCYSASPGLU
5   ASNGLYSERLYSGLYTYRGLYPHEVALHIS
6   PHEGLUTHRGLNGLUALAALAGLUARGALA
7   ILEGLULYSMETASNGLYMETLEULEUASN
8   ASPARGLYSVALPHEVALGLYARGPHELYS
9   SERARGLYSGLUARGGLUALAGLULEUGLY
10   ALAARGALALYSGLUPHETHRASNVALTYR
11   ILELYSASNPHEGLYGLUASPMETASPASP
12   GLUARGLEULYSASPLEUPHEGLYLYSPHE
13   GLYPROALALEUSERVALLYSVALMETTHR
14   ASPGLUSERGLYLYSSERLYSGLYPHEGLY
15   PHEVALSERPHEGLUARGHISGLUASPALA
16   GLNLYSALAVALASPGLUMETASNGLYLYS
17   GLULEUASNGLYLYSGLNILETYRVALGLY
18   ARGALAGLNLYSLYSVALGLUARGGLNTHR
19   GLULEULYSARGLYSPHEGLUGLNMETLYS
20   GLNASPARGILETHR

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks