BMRB Entry 26517
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26517
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Title: TAM DOMAIN OF TIP5_BAZ2A
Deposition date: 2015-02-23 Original release date: 2015-04-28
Authors: Anosova, I.; Melnik, S.; Tripsianes, Kostas; Kateb, F.; Grummt, I.; Sattler, M.
Citation: Anosova, I.; Melnik, S.; Tripsianes, Kostas; Kateb, F.; Grummt, I.; Sattler, M.. "A novel RNA binding surface of the TAM domain of TIP5/BAZ2A mediates epigenetic regulation of rRNA genes" Nucleic Acids Res. ., .-. (2015).
Assembly members:
entity_1, polymer, 109 residues, 13011.8499 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI
Entity Sequences (FASTA):
entity_1: RRRIATPEEVRLPLQHGWRR
EVRIKKGSHRWQGETWYYGP
CGKRMKQFPEVIKYLSRNVV
HSVRREHFSFSPRMPVGDFF
EERDTPEGLQWVQLSAEEIP
SRIQAITGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 392 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 788 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TAM DOMAIN OF TIP5_BAZ2A | 1 |
Entities:
Entity 1, TAM DOMAIN OF TIP5_BAZ2A 109 residues - 13011.8499 Da.
| 1 | ARG | ARG | ARG | ILE | ALA | THR | PRO | GLU | GLU | VAL | ||||
| 2 | ARG | LEU | PRO | LEU | GLN | HIS | GLY | TRP | ARG | ARG | ||||
| 3 | GLU | VAL | ARG | ILE | LYS | LYS | GLY | SER | HIS | ARG | ||||
| 4 | TRP | GLN | GLY | GLU | THR | TRP | TYR | TYR | GLY | PRO | ||||
| 5 | CYS | GLY | LYS | ARG | MET | LYS | GLN | PHE | PRO | GLU | ||||
| 6 | VAL | ILE | LYS | TYR | LEU | SER | ARG | ASN | VAL | VAL | ||||
| 7 | HIS | SER | VAL | ARG | ARG | GLU | HIS | PHE | SER | PHE | ||||
| 8 | SER | PRO | ARG | MET | PRO | VAL | GLY | ASP | PHE | PHE | ||||
| 9 | GLU | GLU | ARG | ASP | THR | PRO | GLU | GLY | LEU | GLN | ||||
| 10 | TRP | VAL | GLN | LEU | SER | ALA | GLU | GLU | ILE | PRO | ||||
| 11 | SER | ARG | ILE | GLN | ALA | ILE | THR | GLY | SER |
Samples:
sample_new_1: TAM, [U-13C; U-15N], 0.6 mM; sodium phosphate 20 mM; NACL 50 mM; DTT 1 mM
sample_conditions_new_1: ionic strength: 100.000 mM; pH: 7.000; pressure: 1.000 atm; temperature: 293.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | sample_new_1 | solution | sample_conditions_new_1 |
| 13C-HSQC | sample_new_1 | solution | sample_conditions_new_1 |
| HNCACB | sample_new_1 | solution | sample_conditions_new_1 |
| HN(CO)CACB | sample_new_1 | solution | sample_conditions_new_1 |
| CC(CO)NH-TOCSY | sample_new_1 | solution | sample_conditions_new_1 |
| HCCH-TOCSY | sample_new_1 | solution | sample_conditions_new_1 |
| 15N-EDITED 3D NOESY | sample_new_1 | solution | sample_conditions_new_1 |
| 13C-EDITED 3D NOESY (ALIPHATICS) | sample_new_1 | solution | sample_conditions_new_1 |
| 13C-EDITED 3D NOESY (AROMATICS) | sample_new_1 | solution | sample_conditions_new_1 |
Software:
AutoDep v4.3, PDBe - chemical shift assignment
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
CYANA v3.0, Guntert, Mumenthaler and Wuthrich - chemical shift assignment
CcpNmr_Analysis v2.3, CCPN - chemical shift assignment, data analysis
SPARKY vany, Goddard - chemical shift assignment
NMR spectrometers:
- BRUKER AVANCE 900 MHz
Related Database Links:
| UNP | BAZ2A_HUMAN |
| PDB | |
| DBJ | BAA20773 BAA89211 BAC31656 BAE21532 BAE33307 |
| EMBL | CAC69992 CAH18232 |
| GB | AAB60864 AAH20083 AAH38012 AAH48415 AAI48496 |
| REF | NP_001100628 NP_001180159 NP_001287834 NP_038477 NP_473419 |
| SP | Q91YE5 Q9UIF9 |
| TPG | DAA29585 |
| AlphaFold | Q96H26 Q9UIF9 Q91YE5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts