BMRB Entry 26554
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26554
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Title: Chemical shift assignments of K2 dehydrin in the presence of SDS micelles PubMed: 26370084
Deposition date: 2015-04-11 Original release date: 2017-08-24
Authors: Graether, Steffen; Warnica, Josephine; Atkinson, John
Citation: Clarke, Matthew; Boddington, Kelly; Warnica, Josephine; Atkinson, John; McKenna, Sarah; Madge, Jeffrey; Barker, Christine; Graether, Steffen. "Structural and Functional Insights into the Cryoprotection of Membranes by the Intrinsically Disordered Dehydrins" J. Biol. Chem. 290, 26900-26913 (2015).
Assembly members:
K2, polymer, 48 residues, Formula weight is not available
Natural source: Common Name: riverbank grape Taxonomy ID: 96939 Superkingdom: Eukaryota Kingdom: Viridipplantae Genus/species: Vitis riparia
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b
Entity Sequences (FASTA):
K2: MKEKIKERIPGMGRKDEQKQ
TSATSTPGQGQQQKGMMEKI
KEKLPGAH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 150 |
| 15N chemical shifts | 44 |
| 1H chemical shifts | 233 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | K2 | 1 |
Entities:
Entity 1, K2 48 residues - Formula weight is not available
| 1 | MET | LYS | GLU | LYS | ILE | LYS | GLU | ARG | ILE | PRO | ||||
| 2 | GLY | MET | GLY | ARG | LYS | ASP | GLU | GLN | LYS | GLN | ||||
| 3 | THR | SER | ALA | THR | SER | THR | PRO | GLY | GLN | GLY | ||||
| 4 | GLN | GLN | GLN | LYS | GLY | MET | MET | GLU | LYS | ILE | ||||
| 5 | LYS | GLU | LYS | LEU | PRO | GLY | ALA | HIS |
Samples:
sample_1: K2, [U-100% 13C; U-100% 15N], 0.2 – 1.0 mM; NaCl 10 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.010 M; pH: 6.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CCPNMR, Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED - chemical shift assignment
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts