BMRB Entry 26575
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26575
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Title: Gli3_1_90 assignment PubMed: 26475525
Deposition date: 2015-05-08 Original release date: 2016-01-05
Authors: Pierce, Wendy; Grace, Christy; Mittag, Tanja
Citation: Pierce, Wendy; Grace, Christy; Lee, Jihun; Nourse, Amanda; Marzahn, Melissa; Watson, Edmond; High, Anthony; Peng, Junmin; Schulman, Brenda; Mittag, Tanja. "Multiple weak linear motifs enhance recruitment and processivity in SPOP-mediated substrate ubiquitination" J. Mol. Biol. 428, 1256-1271 (2016).
Assembly members:
Gli3_1_90, polymer, 90 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
Gli3_1_90: MEAQSHSSTTTEKKKVENSI
VKCSTRTDVSEKAVASSTTS
NEDESPGQTYHRERRNAITM
QPQNVQGLSKVSEEPSTSSD
ERASLIKKEI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 256 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 86 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Gli3_1_90 | 1 |
Entities:
Entity 1, Gli3_1_90 90 residues - Formula weight is not available
| 1 | MET | GLU | ALA | GLN | SER | HIS | SER | SER | THR | THR | |
| 2 | THR | GLU | LYS | LYS | LYS | VAL | GLU | ASN | SER | ILE | |
| 3 | VAL | LYS | CYS | SER | THR | ARG | THR | ASP | VAL | SER | |
| 4 | GLU | LYS | ALA | VAL | ALA | SER | SER | THR | THR | SER | |
| 5 | ASN | GLU | ASP | GLU | SER | PRO | GLY | GLN | THR | TYR | |
| 6 | HIS | ARG | GLU | ARG | ARG | ASN | ALA | ILE | THR | MET | |
| 7 | GLN | PRO | GLN | ASN | VAL | GLN | GLY | LEU | SER | LYS | |
| 8 | VAL | SER | GLU | GLU | PRO | SER | THR | SER | SER | ASP | |
| 9 | GLU | ARG | ALA | SER | LEU | ILE | LYS | LYS | GLU | ILE |
Samples:
sample_1: Gli3_1_90, [U-100% 13C; U-100% 15N], 1.5 mM; potassium chloride 2.7 mM; sodium chloride 50.0 mM; potassium phosphate 1.76 mM; sodium phosphate 10.0 mM; DTT 5.0 mM; D2O 10%; H2O 90%
sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
CARA, Keller and Wuthrich - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts