BMRB Entry 26637

Name: Backbone assignments of dengue virus NS4B N-terminal region
Published: 2016-07-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26637

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of dengue virus NS4B N-terminal region

Deposition date:

2015-08-17

Original release date:

2016-07-15

Authors:

Li, Yan; Kim, Young Mee; Gayen, Shovanlal; Wong, Ying Lei; Huang, Qiwei; Kang, CongBao

Citation:

Citation: Li, Yan; Kim, Young Mee; Zou, Jing; Wang, Qing-Yin; Gayen, Shovanlal; Wong, Ying Lei; Lee, Le Tian; Xie, Xuping; Huang, Qiwei; Lescar, Julien; Shi, Pei-Yong; Kang, CongBao. "Secondary structure and membrane topology of dengue virus NS4B N-terminal 125 amino acids" Biochim. Biophys. Acta 1848, 3150-3157 (2015).
PubMed: 26403837

Assembly members:

Assembly members:
dengue_virus_NS4B_N-terminal_125_amino_acids, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Dengue virus Taxonomy ID: 12637 Superkingdom: Viruses Kingdom: not available Genus/species: Dengue virus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dengue_virus_NS4B_N-terminal_125_amino_acids: NEMGFLEKTKKDFGLGSIAT QQPESNILDIDLRPASAWTL YAVATTFITPMLRHSIENSS VNVSLTAIANQATVLMGLGK GWPLSKMDIGVPLLAIGCYS QVNPITLTAALLLLVAHYAI IGPGLEHHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	505
15N chemical shifts	119
1H chemical shifts	445

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dengue virus NS4B N-terminal 125 amino acids	1

Entities:

Entity 1, dengue virus NS4B N-terminal 125 amino acids 133 residues - Formula weight is not available

1

ASN

GLU

MET

GLY

PHE

LEU

GLU

LYS

THR

LYS

2

LYS

ASP

PHE

GLY

LEU

GLY

SER

ILE

ALA

THR

3

GLN

PRO

GLU

SER

ASN

ILE

LEU

ASP

ILE

4

ASP

LEU

ARG

PRO

ALA

SER

ALA

TRP

THR

LEU

5

TYR

ALA

VAL

ALA

THR

PHE

ILE

THR

PRO

6

MET

LEU

ARG

HIS

SER

ILE

GLU

ASN

SER

7

VAL

ASN

VAL

SER

LEU

THR

ALA

ILE

ALA

ASN

8

GLN

ALA

THR

VAL

LEU

MET

GLY

LEU

GLY

LYS

9

GLY

TRP

PRO

LEU

SER

LYS

MET

ASP

ILE

GLY

10

VAL

PRO

LEU

ALA

ILE

GLY

CYS

TYR

SER

11

GLN

VAL

ASN

PRO

ILE

THR

LEU

THR

ALA

12

LEU

VAL

ALA

HIS

TYR

ALA

ILE

13

ILE

GLY

PRO

GLY

LEU

GLU

HIS

14

HIS

Samples:

sample_1: dengue virus NS4B N-terminal 125 amino acids, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 20 mM; LMPG 1%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment, processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks