BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26645

Title: Backbone 1H, 15N, 13C NMR assignment of the 518-627 fragment of the androgen receptor encompassing N-terminal and DNA Binding domains   PubMed: 26732902

Deposition date: 2015-09-01 Original release date: 2016-07-15

Authors: Kieffer, Bruno; Meyer, Sandra; Perez Escriva, Pau; Wang, Ying-Hui

Citation: Meyer, Sandra; Wang, Ying-Hui; Perez Escriva, Pau; Kieffer, Bruno. "Backbone 1H, 15N, 13C NMR assignment of the 518-627 fragment of the androgen receptor encompassing N-terminal and DNA binding domains"  J. Biomol. NMR 10, 175-178 (2016).

Assembly members:
AR_NTD_DBD_518-627, polymer, 114 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM41

Entity Sequences (FASTA):
AR_NTD_DBD_518-627: GAMGCVKSEMGPWMDSYSGP YGDMRLETARDHVLPIDYYF PPQKTCLICGDEASGCHYGA LTCGSCKVFFKRAAEGKQKY LCASRNDCTIDKFRRKNCPS CRLRKCYEAGMTLG

Data sets:
Data typeCount
13C chemical shifts308
15N chemical shifts104
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AR1
2ZN2

Entities:

Entity 1, AR 114 residues - Formula weight is not available

1   GLYALAMETGLYCYSVALLYSSERGLUMET
2   GLYPROTRPMETASPSERTYRSERGLYPRO
3   TYRGLYASPMETARGLEUGLUTHRALAARG
4   ASPHISVALLEUPROILEASPTYRTYRPHE
5   PROPROGLNLYSTHRCYSLEUILECYSGLY
6   ASPGLUALASERGLYCYSHISTYRGLYALA
7   LEUTHRCYSGLYSERCYSLYSVALPHEPHE
8   LYSARGALAALAGLUGLYLYSGLNLYSTYR
9   LEUCYSALASERARGASNASPCYSTHRILE
10   ASPLYSPHEARGARGLYSASNCYSPROSER
11   CYSARGLEUARGLYSCYSTYRGLUALAGLY
12   METTHRLEUGLY

Entity 2, ZN - 65.409 Da.

1   ZN

Samples:

sample_1: AR NTD DBD 518-627, [U-99% 13C; U-99% 15N], 260 ± 40 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 180 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CCPNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UNP E7EVX6_HUMAN
AlphaFold Q9UD95

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts