BMRB Entry 26653

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Nogo-A-Delta20
Published: 2015-12-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26653

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Nogo-A-Delta20

Deposition date:

2015-09-10

Original release date:

2015-12-09

Authors:

Zelenay, Viviane; Arzt, Michael; Bibow, Stefan; Schwab, Martin; Riek, Roland

Citation:

Citation: Zelenay, Viviane; Arzt, Michael; Bibow, Stefan; Schwab, Martin; Riek, Roland. "The Neurite Outgrowth Inhibitory Nogo-A-Delta20 Region is an Intrinsically Disordered Segment Harbouring Three Stretches with Helical Propensity" PLoS One 11, e0161813-e0161813 (2016).
PubMed: 27611089

Assembly members:

Assembly members:
Nogo-A-Delta20, polymer, 207 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nogo-A-Delta20: GSHMASMTGGQQMGRGSTGT KIAYETKVDLVQTSEAIQES LYPTAQLCPSFEEAEATPSP VLPDIVMEAPLNSLLPSAGA SVVQPSVSPLEAPPPVSYDS IKLEPENPPPYEEAMNVALK ALGTKEGIKEPESFNAAVQE TEAPYISIACDLIKETKLST EPSPDFSNYSEIAKFEKSVP EHAELVEDSSPESEPVDLFL EHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	352
15N chemical shifts	170
1H chemical shifts	237

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nogo-A-Delta20, 1	1
2	Nogo-A-Delta20, 2	1

Entities:

Entity 1, Nogo-A-Delta20, 1 207 residues - Formula weight is not available

1

GLY

SER

HIS

MET

ALA

SER

MET

THR

GLY

2

GLN

MET

GLY

ARG

GLY

SER

THR

GLY

THR

3

LYS

ILE

ALA

TYR

GLU

THR

LYS

VAL

ASP

LEU

4

VAL

GLN

THR

SER

GLU

ALA

ILE

GLN

GLU

SER

5

LEU

TYR

PRO

THR

ALA

GLN

LEU

CYS

PRO

SER

6

PHE

GLU

ALA

GLU

ALA

THR

PRO

SER

PRO

7

VAL

LEU

PRO

ASP

ILE

VAL

MET

GLU

ALA

PRO

8

LEU

ASN

SER

LEU

PRO

SER

ALA

GLY

ALA

9

SER

VAL

GLN

PRO

SER

VAL

SER

PRO

LEU

10

GLU

ALA

PRO

VAL

SER

TYR

ASP

SER

11

ILE

LYS

LEU

GLU

PRO

GLU

ASN

PRO

12

TYR

GLU

ALA

MET

ASN

VAL

ALA

LEU

LYS

13

ALA

LEU

GLY

THR

LYS

GLU

GLY

ILE

LYS

GLU

14

PRO

GLU

SER

PHE

ASN

ALA

VAL

GLN

GLU

15

THR

GLU

ALA

PRO

TYR

ILE

SER

ILE

ALA

CYS

16

ASP

LEU

ILE

LYS

GLU

THR

LYS

LEU

SER

THR

17

GLU

PRO

SER

PRO

ASP

PHE

SER

ASN

TYR

SER

18

GLU

ILE

ALA

LYS

PHE

GLU

LYS

SER

VAL

PRO

19

GLU

HIS

ALA

GLU

LEU

VAL

GLU

ASP

SER

20

PRO

GLU

SER

GLU

PRO

VAL

ASP

LEU

PHE

LEU

21

GLU

HIS

Samples:

sample_1: Nogo-A-Delta20, [U-100% 13C; U-100% 15N], 0.4 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 279 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
HNN	sample_1	isotropic	sample_conditions_1
HCAN	sample_1	isotropic	sample_conditions_1

Software:

CCPNMR, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance III 600 MHz
Bruker Avance III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks