BMRB Entry 26662
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26662
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Title: Backbone 1H, 13C and 15N Chemical Shift Assignments for c-Myc-1-88 PubMed: 26655473
Deposition date: 2015-09-17 Original release date: 2015-12-17
Authors: Helander, Sara; Montecchio, Meri; Sunnerhagen, Maria
Citation: Helander, Sara; Montecchio, Meri; Pilstal, Robert; Su, Yulong; Kuruvilla, Jacob; Elven, Malin; Ziauddin, Javed; Anandapadamanaban, Madhanagopal; Cristobal, Susana; Lundstrom, Patrik; Sears, Rosalie; Wallner, Bjorn; Sunnerhagen, Maria. "Pre-Anchoring of Pin1 to Unphosphorylated c-Myc in a Fuzzy Complex Regulates c-Myc Activity" Structure 23, 2267-2279 (2015).
Assembly members:
c-Myc, polymer, 94 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETMCSIII
Entity Sequences (FASTA):
c-Myc: HHHHHHMPLNVSFTNRNYDL
DYDSVQPYFYCDEEENFYQQ
QQQSELQPPAPSEDIWKKFE
LLPTPPLSPSRRSGLCSPSY
VAVTPFSLRGDNDG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 235 |
| 15N chemical shifts | 76 |
| 1H chemical shifts | 71 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | c-Myc | 1 |
Entities:
Entity 1, c-Myc 94 residues - Formula weight is not available
| 1 | HIS | HIS | HIS | HIS | HIS | HIS | MET | PRO | LEU | ASN | ||||
| 2 | VAL | SER | PHE | THR | ASN | ARG | ASN | TYR | ASP | LEU | ||||
| 3 | ASP | TYR | ASP | SER | VAL | GLN | PRO | TYR | PHE | TYR | ||||
| 4 | CYS | ASP | GLU | GLU | GLU | ASN | PHE | TYR | GLN | GLN | ||||
| 5 | GLN | GLN | GLN | SER | GLU | LEU | GLN | PRO | PRO | ALA | ||||
| 6 | PRO | SER | GLU | ASP | ILE | TRP | LYS | LYS | PHE | GLU | ||||
| 7 | LEU | LEU | PRO | THR | PRO | PRO | LEU | SER | PRO | SER | ||||
| 8 | ARG | ARG | SER | GLY | LEU | CYS | SER | PRO | SER | TYR | ||||
| 9 | VAL | ALA | VAL | THR | PRO | PHE | SER | LEU | ARG | GLY | ||||
| 10 | ASP | ASN | ASP | GLY |
Samples:
sample_1: c-Myc, [U-99% 13C; U-99% 15N], 80 – 250 uM; DTT 5 mM; glycerol 5%; H2O 90%; D2O 10%; HEPES 20 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HACAN | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Analysis of data processing
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts