BMRB Entry 26719

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26719

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Title:
Backbone and triple resonance 1H, 13C, and 15N Chemical Shift Assignments for residues 420-500 of Saccharomyces cerevisiae transcription factor Ash1
Deposition date:
2015-12-11
Original release date:
2018-02-23
Authors:
Martin, Erik; Grace, Christy; Mittag, Tanja
Citation:

Citation: Martin, Erik; Holehouse, Alex; Grace, Christy; Hughes, Alex; Pappu, Rohit; Mittag, Tanja. "Sequence Determinants of the Conformational Properties of an Intrinsically Disordered Protein Prior to and upon Multisite Phosphorylation"  J. Am. Chem. Soc. 138, 15323-15335 (2016).
PubMed: 27807972

Assembly members:

Assembly members:
Ash1_420-500, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ash1_420-500: GASASSSPSPSTPTKSGKMR SRSSSPVRPKAYTPSPRSPN YHRFALDSPPQSPRRSSNSS ITKKGSRRSSGSSPTRHTTR VCV

Data sets:
Data typeCount
13C chemical shifts198
15N chemical shifts70
1H chemical shifts67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ash1 420-5001

Entities:

Entity 1, Ash1 420-500 83 residues - Formula weight is not available

N-terminal Gly-Ala are an artifact from affinity tag cleavage.

1   GLYALASERALASERSERSERPROSERPRO
2   SERTHRPROTHRLYSSERGLYLYSMETARG
3   SERARGSERSERSERPROVALARGPROLYS
4   ALATYRTHRPROSERPROARGSERPROASN
5   TYRHISARGPHEALALEUASPSERPROPRO
6   GLNSERPROARGARGSERSERASNSERSER
7   ILETHRLYSLYSGLYSERARGARGSERSER
8   GLYSERSERPROTHRARGHISTHRTHRARG
9   VALCYSVAL

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks