BMRB Entry 26793
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26793
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Title: Backbone assignment of p24 GOLD domain PubMed: 27569046
Deposition date: 2016-05-10 Original release date: 2016-10-14
Authors: Yamaguchi, Yoshiki; Nagae, Masamichi
Citation: Nagae, Masamichi; Hirata, Tetsuya; Morita-Matsumoto, Kana; Theiler, Romina; Fujita, Morihisa; Kinoshita, Taroh; Yamaguchi, Yoshiki. "3D structure and interaction of p24beta and p24delta Golgi dynamics domains: implications for p24 complex formation and cargo transport" J. Mol. Biol. 428, 4087-4099 (2016).
Assembly members:
p24, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCold
Entity Sequences (FASTA):
p24: GSSGYFVSIDAHAEECFFER
VTSGTKMGLIFEVAEGGFLD
IDVEITGPDNKGIYKGDRES
SGKYTFAAHMDGTYKFCFSN
RMSTMTPKIVMFTIDIGEAP
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 85 |
| 15N chemical shifts | 48 |
| 1H chemical shifts | 48 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GOLD domain | 1 |
Entities:
Entity 1, GOLD domain 101 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | TYR | PHE | VAL | SER | ILE | ASP | ||||
| 2 | ALA | HIS | ALA | GLU | GLU | CYS | PHE | PHE | GLU | ARG | ||||
| 3 | VAL | THR | SER | GLY | THR | LYS | MET | GLY | LEU | ILE | ||||
| 4 | PHE | GLU | VAL | ALA | GLU | GLY | GLY | PHE | LEU | ASP | ||||
| 5 | ILE | ASP | VAL | GLU | ILE | THR | GLY | PRO | ASP | ASN | ||||
| 6 | LYS | GLY | ILE | TYR | LYS | GLY | ASP | ARG | GLU | SER | ||||
| 7 | SER | GLY | LYS | TYR | THR | PHE | ALA | ALA | HIS | MET | ||||
| 8 | ASP | GLY | THR | TYR | LYS | PHE | CYS | PHE | SER | ASN | ||||
| 9 | ARG | MET | SER | THR | MET | THR | PRO | LYS | ILE | VAL | ||||
| 10 | MET | PHE | THR | ILE | ASP | ILE | GLY | GLU | ALA | PRO | ||||
| 11 | LYS |
Samples:
sample_1: p24, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 90%; D2O 10%; sodium phosphate 20 mM; NaCl 50 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts