BMRB Entry 26801

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26801

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Title:
1H, 13C and 15N Assignment of NFU1 Iron-Sulfur Cluster Scaffold Homolog from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR2876
Deposition date:
2016-05-16
Original release date:
2016-06-29
Authors:
Liu, Gaohua; Xiao, Rong; Janjua, Haleema; Hamilton, Keith; Shastry, Ritu; Kohan, Eitan; Acton, Thomas; Everett, John; Huang, Yuangpeng; Montelione, Gaetano
Citation:

Citation: Cai, Kai; Liu, Gaohua; Frederick, Ronnie; Xiao, Rong; Montelione, Gaetano; Markley, John. "Structural/Functional Properties of Human NFU1, an Intermediate [4Fe-4S] Carrier in Human Mitochondrial Iron-Sulfur Cluster Biogenesis"  Structure 24, 2080-2091 (2016).
PubMed: 27818104

Assembly members:

Assembly members:
NFU1, polymer, 216 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NFU1: MGSSHHHHHHSSGLVPRGSH MFIQTQDTPNPNSLKFIPGK PVLETRTMDFPTPAAAFRSP LARQLFRIEGVKSVFFGPDF ITVTKENEELDWNLLKPDIY ATIMDFFASGLPLVTEETPS GEAGSEEDDEVVAMIKELLD TRIRPTVQEDGGDVIYKGFE DGIVQLKLQGSCTSCPSSII TLKNGIQNMLQFYIPEVEGV EQVMDDESDEKEANSP

Data sets:
Data typeCount
13C chemical shifts680
15N chemical shifts196
1H chemical shifts1380

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NFU11

Entities:

Entity 1, NFU1 216 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METPHEILEGLNTHRGLNASPTHRPROASN
4   PROASNSERLEULYSPHEILEPROGLYLYS
5   PROVALLEUGLUTHRARGTHRMETASPPHE
6   PROTHRPROALAALAALAPHEARGSERPRO
7   LEUALAARGGLNLEUPHEARGILEGLUGLY
8   VALLYSSERVALPHEPHEGLYPROASPPHE
9   ILETHRVALTHRLYSGLUASNGLUGLULEU
10   ASPTRPASNLEULEULYSPROASPILETYR
11   ALATHRILEMETASPPHEPHEALASERGLY
12   LEUPROLEUVALTHRGLUGLUTHRPROSER
13   GLYGLUALAGLYSERGLUGLUASPASPGLU
14   VALVALALAMETILELYSGLULEULEUASP
15   THRARGILEARGPROTHRVALGLNGLUASP
16   GLYGLYASPVALILETYRLYSGLYPHEGLU
17   ASPGLYILEVALGLNLEULYSLEUGLNGLY
18   SERCYSTHRSERCYSPROSERSERILEILE
19   THRLEULYSASNGLYILEGLNASNMETLEU
20   GLNPHETYRILEPROGLUVALGLUGLYVAL
21   GLUGLNVALMETASPASPGLUSERASPGLU
22   LYSGLUALAASNSERPRO

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