BMRB Entry 26803

Name: Recombinant human carcinoembryonic antigen related cell adhesion molecule 1 (hCEACAM1) N-terminal Igv domain
Published: 2016-08-31

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26803

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Recombinant human carcinoembryonic antigen related cell adhesion molecule 1 (hCEACAM1) N-terminal Igv domain

Deposition date:

2016-05-18

Original release date:

2016-08-31

Authors:

Prestegard, James; Zhuo, You

Citation:

Citation: Zhuo, You; Yang, Jeong-Yeh; Moremen, Kelley; Prestegard, James. "Glycosylation Alters Dimerization Properties of a Cell-surface Signaling Protein, Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1)" J. Biol. Chem. 291, 20085-20095 (2016).
PubMed: 27471271

Assembly members:

Assembly members:
CEACAM1-Igv, polymer, 120 residues, 13371.78 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CEACAM1-Igv: MAQLTTESMPFNVAEGKEVL LLVHNLPQQLFGYSWYKGER VDGNRQIVGYAIGTQQATPG PANSGRETIYPNASLLIQNV TQNDTGFYTLQVIKSDLVNE EATGQFHVYAAALEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	247
15N chemical shifts	83
1H chemical shifts	83

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CEACAM1-Igv, chain 1	1
2	CEACAM1-Igv, chain 2	1

Entities:

Entity 1, CEACAM1-Igv, chain 1 120 residues - 13371.78 Da.

Residues 110-120 represent a non-native His-tag.

1	MET	ALA	GLN	LEU	THR	THR	GLU	SER	MET	PRO
2	PHE	ASN	VAL	ALA	GLU	GLY	LYS	GLU	VAL	LEU
3	LEU	LEU	VAL	HIS	ASN	LEU	PRO	GLN	GLN	LEU
4	PHE	GLY	TYR	SER	TRP	TYR	LYS	GLY	GLU	ARG
5	VAL	ASP	GLY	ASN	ARG	GLN	ILE	VAL	GLY	TYR
6	ALA	ILE	GLY	THR	GLN	GLN	ALA	THR	PRO	GLY
7	PRO	ALA	ASN	SER	GLY	ARG	GLU	THR	ILE	TYR
8	PRO	ASN	ALA	SER	LEU	LEU	ILE	GLN	ASN	VAL
9	THR	GLN	ASN	ASP	THR	GLY	PHE	TYR	THR	LEU
10	GLN	VAL	ILE	LYS	SER	ASP	LEU	VAL	ASN	GLU
11	GLU	ALA	THR	GLY	GLN	PHE	HIS	VAL	TYR	ALA
12	ALA	ALA	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 26803

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	MET	ALA	GLN	LEU	THR	THR	GLU	SER	MET	PRO
2	PHE	ASN	VAL	ALA	GLU	GLY	LYS	GLU	VAL	LEU
3	LEU	LEU	VAL	HIS	ASN	LEU	PRO	GLN	GLN	LEU
4	PHE	GLY	TYR	SER	TRP	TYR	LYS	GLY	GLU	ARG
5	VAL	ASP	GLY	ASN	ARG	GLN	ILE	VAL	GLY	TYR
6	ALA	ILE	GLY	THR	GLN	GLN	ALA	THR	PRO	GLY
7	PRO	ALA	ASN	SER	GLY	ARG	GLU	THR	ILE	TYR
8	PRO	ASN	ALA	SER	LEU	LEU	ILE	GLN	ASN	VAL
9	THR	GLN	ASN	ASP	THR	GLY	PHE	TYR	THR	LEU
10	GLN	VAL	ILE	LYS	SER	ASP	LEU	VAL	ASN	GLU
11	GLU	ALA	THR	GLY	GLN	PHE	HIS	VAL	TYR	ALA
12	ALA	ALA	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	ALA	GLN	LEU	THR	THR	GLU	SER	MET	PRO
2	PHE	ASN	VAL	ALA	GLU	GLY	LYS	GLU	VAL	LEU
3	LEU	LEU	VAL	HIS	ASN	LEU	PRO	GLN	GLN	LEU
4	PHE	GLY	TYR	SER	TRP	TYR	LYS	GLY	GLU	ARG
5	VAL	ASP	GLY	ASN	ARG	GLN	ILE	VAL	GLY	TYR
6	ALA	ILE	GLY	THR	GLN	GLN	ALA	THR	PRO	GLY
7	PRO	ALA	ASN	SER	GLY	ARG	GLU	THR	ILE	TYR
8	PRO	ASN	ALA	SER	LEU	LEU	ILE	GLN	ASN	VAL
9	THR	GLN	ASN	ASP	THR	GLY	PHE	TYR	THR	LEU
10	GLN	VAL	ILE	LYS	SER	ASP	LEU	VAL	ASN	GLU
11	GLU	ALA	THR	GLY	GLN	PHE	HIS	VAL	TYR	ALA
12	ALA	ALA	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	ALA	GLN	LEU	THR	THR	GLU	SER	MET	PRO
2	PHE	ASN	VAL	ALA	GLU	GLY	LYS	GLU	VAL	LEU
3	LEU	LEU	VAL	HIS	ASN	LEU	PRO	GLN	GLN	LEU
4	PHE	GLY	TYR	SER	TRP	TYR	LYS	GLY	GLU	ARG
5	VAL	ASP	GLY	ASN	ARG	GLN	ILE	VAL	GLY	TYR
6	ALA	ILE	GLY	THR	GLN	GLN	ALA	THR	PRO	GLY
7	PRO	ALA	ASN	SER	GLY	ARG	GLU	THR	ILE	TYR
8	PRO	ASN	ALA	SER	LEU	LEU	ILE	GLN	ASN	VAL
9	THR	GLN	ASN	ASP	THR	GLY	PHE	TYR	THR	LEU
10	GLN	VAL	ILE	LYS	SER	ASP	LEU	VAL	ASN	GLU
11	GLU	ALA	THR	GLY	GLN	PHE	HIS	VAL	TYR	ALA
12	ALA	ALA	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS