BMRB Entry 26843
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26843
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for NCP15 nucleocapsid PubMed: 29332151
Deposition date: 2016-07-04 Original release date: 2016-08-23
Authors: LARUE, Valery; TISNE, Carine
Citation: Larue, Valery; Catala, Marjorie; Belfetmi, Anissa; Zargarian, Loussine; Mauffret, Olivier; Tisne, Carine. "." Biomol. NMR Assign. 12, 139-143 (2018).
Assembly members:
HIV-1_NCP15_Nucleocapsid, polymer, 123 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: HIV Taxonomy ID: 12721 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a
Entity Sequences (FASTA):
HIV-1_NCP15_Nucleocapsid: IQKGNFRNQRKTVKCFNCGK
EGHIAKNCRAPRKKGCWKCG
KEGHQMKDCTERQANFLGKI
WPSHKGRPGNFLQSRPEPTA
PPEESFRFGEETTTPSQKQE
PIDKELYPLASLRSLFGSDP
SSQ
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 296 |
15N chemical shifts | 104 |
1H chemical shifts | 283 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NCP15 | 1 |
2 | ZnCl2 | 2 |
Entities:
Entity 1, NCP15 123 residues - Formula weight is not available
1 | ILE | GLN | LYS | GLY | ASN | PHE | ARG | ASN | GLN | ARG | ||||
2 | LYS | THR | VAL | LYS | CYS | PHE | ASN | CYS | GLY | LYS | ||||
3 | GLU | GLY | HIS | ILE | ALA | LYS | ASN | CYS | ARG | ALA | ||||
4 | PRO | ARG | LYS | LYS | GLY | CYS | TRP | LYS | CYS | GLY | ||||
5 | LYS | GLU | GLY | HIS | GLN | MET | LYS | ASP | CYS | THR | ||||
6 | GLU | ARG | GLN | ALA | ASN | PHE | LEU | GLY | LYS | ILE | ||||
7 | TRP | PRO | SER | HIS | LYS | GLY | ARG | PRO | GLY | ASN | ||||
8 | PHE | LEU | GLN | SER | ARG | PRO | GLU | PRO | THR | ALA | ||||
9 | PRO | PRO | GLU | GLU | SER | PHE | ARG | PHE | GLY | GLU | ||||
10 | GLU | THR | THR | THR | PRO | SER | GLN | LYS | GLN | GLU | ||||
11 | PRO | ILE | ASP | LYS | GLU | LEU | TYR | PRO | LEU | ALA | ||||
12 | SER | LEU | ARG | SER | LEU | PHE | GLY | SER | ASP | PRO | ||||
13 | SER | SER | GLN |
Entity 2, ZnCl2 - 65.409 Da.
1 | ZN |
Samples:
sample_1: NCP15 Nucleocapsid, [U-100% 13C; U-100% 15N], 0.8 mM; acetate buffer 25 mM; NaCl 25 mM; ZnCl2 0.1 mM; Mercaptoethanol 0.1 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 950 MHz
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts