BMRB Entry 26845
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26845
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Title: Field dependent R1 and R2 relaxation in B3 domain of protein G (GB3) PubMed: 27734179
Deposition date: 2016-07-05 Original release date: 2016-12-29
Authors: Hernandez, Griselda; LeMaster, David
Citation: Hernandez, Griselda; LeMaster, David. "Quantifying protein dynamics in the ps-ns time regime by NMR relaxation" J. Biomol. NMR 66, 163-174 (2016).
Assembly members:
GB3, polymer, 56 residues, 6207.8 Da.
Natural source: Common Name: Streptococcus Taxonomy ID: 1301 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
GB3: MQYKLVINGKTLKGETTTKA
VDAETAEKAFKQYANDNGVD
GVWTYDDATKTFTVTE
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| T1 relaxation values | 204 |
| T2 relaxation values | 204 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GB3 | 1 |
Entities:
Entity 1, GB3 56 residues - 6207.8 Da.
| 1 | MET | GLN | TYR | LYS | LEU | VAL | ILE | ASN | GLY | LYS | ||||
| 2 | THR | LEU | LYS | GLY | GLU | THR | THR | THR | LYS | ALA | ||||
| 3 | VAL | ASP | ALA | GLU | THR | ALA | GLU | LYS | ALA | PHE | ||||
| 4 | LYS | GLN | TYR | ALA | ASN | ASP | ASN | GLY | VAL | ASP | ||||
| 5 | GLY | VAL | TRP | THR | TYR | ASP | ASP | ALA | THR | LYS | ||||
| 6 | THR | PHE | THR | VAL | THR | GLU |
Samples:
sample_1: GB3, [U-99% 15N], 2 mM; sodium phosphate 25 mM; sodium azide 0.01%
sample_conditions_1: ionic strength: 30 mM; pH: 6.3; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| T1_relaxation_500 | sample_1 | isotropic | sample_conditions_1 |
| T1_relaxation_600 | sample_1 | isotropic | sample_conditions_1 |
| T1_relaxation_800 | sample_1 | isotropic | sample_conditions_1 |
| T1_relaxation_900 | sample_1 | isotropic | sample_conditions_1 |
| T2_relaxation_500 | sample_1 | isotropic | sample_conditions_1 |
| T2_relaxation_600 | sample_1 | isotropic | sample_conditions_1 |
| T2_relaxation_800 | sample_1 | isotropic | sample_conditions_1 |
| T2_relaxation_900 | sample_1 | isotropic | sample_conditions_1 |
Software:
Felix, Felix NMR - data analysis
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz