BMRB Entry 26861

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26861

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Title:
Tunicate_crystallin_Ca2+form
Deposition date:
2016-07-26
Original release date:
2017-02-16
Authors:
Kozlyuk, Natalia; Martin, Rachel
Citation:

Citation: Kozlyuk, Natalia; Sengupta, Suvrajit; Bierma, Jan; Martin, Rachel. "Calcium Binding Dramatically Stabilizes an Ancestral Crystallin Fold in tunicate beta gamma crystallin"  Biochemistry 55, 6961-6968 (2016).
PubMed: 27992995

Assembly members:

Assembly members:
Tunicate, polymer, 84 residues, 9057 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tunicate: MGKIILFEDVEFGGKKLELE TSVSDLNVHGFNDIVSSIIV ESGTWFVFDDEGFSGPSYKL TPGKYPNPGSWGGNDDELSS VKQQ

Data sets:
Data typeCount
13C chemical shifts225
15N chemical shifts79
1H chemical shifts79

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tunicate beta gamma crystallin1
2Calcium ion2

Entities:

Entity 1, Tunicate beta gamma crystallin 84 residues - 9057 Da.

1   METGLYLYSILEILELEUPHEGLUASPVAL
2   GLUPHEGLYGLYLYSLYSLEUGLULEUGLU
3   THRSERVALSERASPLEUASNVALHISGLY
4   PHEASNASPILEVALSERSERILEILEVAL
5   GLUSERGLYTHRTRPPHEVALPHEASPASP
6   GLUGLYPHESERGLYPROSERTYRLYSLEU
7   THRPROGLYLYSTYRPROASNPROGLYSER
8   TRPGLYGLYASNASPASPGLULEUSERSER
9   VALLYSGLNGLN

Entity 2, Calcium ion - 40.078 Da.

1   CA

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