BMRB Entry 26884

Name: Backbone assignment of Rhea (talin) F0.
Published: 2018-11-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26884

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of Rhea (talin) F0.

Deposition date:

2016-09-01

Original release date:

2018-11-26

Authors:

Castle, Will; Tanentzapf, Guy; Goult, Ben

Citation:

Citation: Camp, D; Haage, A; Solianova, V; Castle, W; Xu, Q; Lostchuck, E; Goult, B; Tanentzapf, G. "Direct binding of Talin to Rap1 is required for cell-ECM adhesion in Drosophila" J. Cell. Sci. 131, 225144-225144 (2018).
PubMed: 30446511

Assembly members:

Assembly members:
Rhea, polymer, 89 residues, 10243.5 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLEICS-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rhea: SMMSTLSLRIQLEGGRVTKT IQFQPNTTVFDACKVIRDKF AEAVQGQPSEYGLFISDEQN QQGVWLEPGRTLGYYILHNQ DTLEYRRKT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	245
15N chemical shifts	83
1H chemical shifts	83

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rhea F0	1

Entities:

Entity 1, rhea F0 89 residues - 10243.5 Da.

Residues 1-2 represent a non-native affinity tag

1

SER

MET

SER

THR

LEU

SER

LEU

ARG

ILE

2

GLN

LEU

GLU

GLY

ARG

VAL

THR

LYS

THR

3

ILE

GLN

PHE

GLN

PRO

ASN

THR

VAL

PHE

4

ASP

ALA

CYS

LYS

VAL

ILE

ARG

ASP

LYS

PHE

5

ALA

GLU

ALA

VAL

GLN

GLY

GLN

PRO

SER

GLU

6

TYR

GLY

LEU

PHE

ILE

SER

ASP

GLU

GLN

ASN

7

GLN

GLY

VAL

TRP

LEU

GLU

PRO

GLY

ARG

8

THR

LEU

GLY

TYR

ILE

LEU

HIS

ASN

GLN

9

ASP

THR

LEU

GLU

TYR

ARG

LYS

THR

Samples:

15N_labelled: Rhea, [U-100% 15N], 100 ± 1 uM; D2O, [U-100% 2H], 5 ± 0.1 %; sodium phosphate 20 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; DTT 2 ± 0.1 mM

double_labelled: Rhea, [U-100% 13C; U-100% 15N], 500 ± 1 uM; D2O, [U-100% 2H], 5 ± 0.1 %; sodium phosphate 20 ± 0.1 mM; sodium chloride 50 ± 0.1 mM; DTT 2 ± 0.1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N_labelled	isotropic	sample_conditions_1
3D CBCA(CO)NH	double_labelled	isotropic	sample_conditions_1
3D HNCO	double_labelled	isotropic	sample_conditions_1
3D HNCA	double_labelled	isotropic	sample_conditions_1
3D HNCACB	double_labelled	isotropic	sample_conditions_1
3D HN(CO)CA	double_labelled	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CCPNMR_Analysis v2.15, CCPN - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks