BMRB Entry 26900
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26900
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Title: Beta-endorphin salt-free amyloid fibrils PubMed: 28075583
Deposition date: 2016-09-16 Original release date: 2021-07-22
Authors: Seuring, Carolin; Verasdonck, Joeri; Ringler, Philippe; Cadalbert, Riccardo; Stahlberg, Henning; Boeckmann, Anja; Meier, Beat; Riek, Roland
Citation: Seuring, Carolin; Verasdonck, Joeri; Ringler, Philippe; Cadalbert, Riccardo; Stahlberg, Henning; Boeckmann, Anja; Meier, Beat; Riek, Roland. "Amyloid Fibril Polymorphism: Almost Identical on the Atomic Level, Mesoscopically Very Different" J Phys Chem B 121, 1783-1792 (2017).
Assembly members:
beta-endorphin, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-32a
Entity Sequences (FASTA):
beta-endorphin: YGGFMTSEKSQTPLVTLFKN
AIIKNAYKKE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 122 |
| 15N chemical shifts | 28 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | beta-endorphin amyloid fibril | 1 |
Entities:
Entity 1, beta-endorphin amyloid fibril 30 residues - Formula weight is not available
| 1 | TYR | GLY | GLY | PHE | MET | THR | SER | GLU | LYS | SER | |
| 2 | GLN | THR | PRO | LEU | VAL | THR | LEU | PHE | LYS | ASN | |
| 3 | ALA | ILE | ILE | LYS | ASN | ALA | TYR | LYS | LYS | GLU |
Samples:
sample_1: beta-endorphin, [U-100% 13C; U-100% 15N], 25 mg
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 20 ms DAR | sample_1 | isotropic | sample_conditions_1 |
| 2D NCA | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCA | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, CCPN - collection, data analysis, processing
NMR spectrometers:
- Bruker Avance 600 MHz