BMRB Entry 26906

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26906

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Title:
Backbone chemical shift assignment of the C-terminal domain of Respiratory Syncytial Virus phosphoprotein
Deposition date:
2016-09-22
Original release date:
2017-02-15
Authors:
Lassoued, Safa; Pereira, Nelson; Fix, Jenna; Galloux, Marie; Sizun, Christina; Eleouet, Jean-Francois
Citation:

Citation: Pereira, Nelson; Cardone, Christophe; Lassoued, Safa; Galloux, Marie; Fix, Jenna; Assrir, Nadine; Lescop, Ewen; Bontems, Francois; Eleouet, Jean-Francois; Sizun, Christina. "New Insights into Structural Disorder in Human Respiratory Syncytial Virus Phosphoprotein and Implications for Binding of Protein Partners"  J. Biol. Chem. 292, 2120-2131 (2017).
PubMed: 28031463

Assembly members:

Assembly members:
P-CTD, polymer, 83 residues, 9323.1498 Da.

Natural source:

Natural source:   Common Name: human RSV   Taxonomy ID: 11250   Superkingdom: Viruses   Kingdom: not available   Genus/species: Orthopneumovirus HRSV

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
P-CTD: GSSARDGIRDAMVGLREEMI EKIRTEALMTNDRLEAMARL RNEESEKMAKDTSDEVSLNP TSEKLNNLLEGNDSDNDLSL EDF

Data sets:
Data typeCount
13C chemical shifts238
15N chemical shifts81
1H chemical shifts159

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1P-CTD1

Entities:

Entity 1, P-CTD 83 residues - 9323.1498 Da.

P-CTD is a phosphoprotein fragment corresponding to the C-terminal domains of hRSV (residues S161-DF241). The two N-terminal residues (GS) are left over from a cleaved GST-tag.

1   GLYSERSERALAARGASPGLYILEARGASP
2   ALAMETVALGLYLEUARGGLUGLUMETILE
3   GLULYSILEARGTHRGLUALALEUMETTHR
4   ASNASPARGLEUGLUALAMETALAARGLEU
5   ARGASNGLUGLUSERGLULYSMETALALYS
6   ASPTHRSERASPGLUVALSERLEUASNPRO
7   THRSERGLULYSLEUASNASNLEULEUGLU
8   GLYASNASPSERASPASNASPLEUSERLEU
9   GLUASPPHE

Related Database Links:

UniProt P12579
AlphaFold P12579

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks