BMRB Entry 26931

Name: Backbone chemical shifts of the MBD2/3 methyl-cytosine binding domain from Ephydatia muelleri
Published: 2021-07-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26931

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shifts of the MBD2/3 methyl-cytosine binding domain from Ephydatia muelleri

Deposition date:

2016-11-01

Original release date:

2021-07-22

Authors:

Williams, David; Bilinovich, Stephanie

Citation:

Citation: Cramer, Jason; Pohlmann, Deborah; Gomez, Fernando; Mark, Leslie; Kornegay, Benjamin; Hall, Chelsea; Siraliev-Perez, Edhriz; Walavalkar, Ninad; Sperlazza, Jeannette; Bilinovich, Stephanie; Prokop, Jeremy; Hill, April; Williams, David. "Methylation specific targeting of a chromatin remodeling complex from sponges to humans" Sci. Rep. 7, 40674-40674 (2017).
PubMed: 28094816

Assembly members:

Assembly members:
EmMBD2, polymer, 70 residues, Formula weight is not available
1xmCpG-forward, polymer, 17 residues, Formula weight is not available
1xmCpG-reverse, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source: Common Name: sponges Taxonomy ID: 6052 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ephydatia muelleri

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EmMBD2: GSLRVKGLPSGWKREVVVRK NGQSAGKTDVYYFSPCGKKF RSKPQIARFLGDAVDLTCFD FSRAGSPGDG
1xmCpG-forward: GAGGCGCTXGGCGGCAG
1xmCpG-reverse: CTGCCGCXGAGCGCCTC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	180
15N chemical shifts	62
1H chemical shifts	62

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EmMBD2	1
2	DNA, chain 1	2
3	DNA, chain 2	3

Entities:

Entity 1, EmMBD2 70 residues - Formula weight is not available

1	GLY	SER	LEU	ARG	VAL	LYS	GLY	LEU	PRO	SER
2	GLY	TRP	LYS	ARG	GLU	VAL	VAL	VAL	ARG	LYS
3	ASN	GLY	GLN	SER	ALA	GLY	LYS	THR	ASP	VAL
4	TYR	TYR	PHE	SER	PRO	CYS	GLY	LYS	LYS	PHE
5	ARG	SER	LYS	PRO	GLN	ILE	ALA	ARG	PHE	LEU
6	GLY	ASP	ALA	VAL	ASP	LEU	THR	CYS	PHE	ASP
7	PHE	SER	ARG	ALA	GLY	SER	PRO	GLY	ASP	GLY

Entity 2, DNA, chain 1 17 residues - Formula weight is not available

X=mC

1

DG

DA

DG

DC

DG

DC

DT

3MC

DG

2

DG

DC

DG

DC

DA

DG

Entity 3, DNA, chain 2 17 residues - Formula weight is not available

X=mC

1

DC

DT

DG

DC

DG

DC

3MC

DG

DA

2

DG

DC

DG

DC

DT

DC

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 26931

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	SER	LEU	ARG	VAL	LYS	GLY	LEU	PRO	SER
2	GLY	TRP	LYS	ARG	GLU	VAL	VAL	VAL	ARG	LYS
3	ASN	GLY	GLN	SER	ALA	GLY	LYS	THR	ASP	VAL
4	TYR	TYR	PHE	SER	PRO	CYS	GLY	LYS	LYS	PHE
5	ARG	SER	LYS	PRO	GLN	ILE	ALA	ARG	PHE	LEU
6	GLY	ASP	ALA	VAL	ASP	LEU	THR	CYS	PHE	ASP
7	PHE	SER	ARG	ALA	GLY	SER	PRO	GLY	ASP	GLY

1	GLY	SER	LEU	ARG	VAL	LYS	GLY	LEU	PRO	SER
2	GLY	TRP	LYS	ARG	GLU	VAL	VAL	VAL	ARG	LYS
3	ASN	GLY	GLN	SER	ALA	GLY	LYS	THR	ASP	VAL
4	TYR	TYR	PHE	SER	PRO	CYS	GLY	LYS	LYS	PHE
5	ARG	SER	LYS	PRO	GLN	ILE	ALA	ARG	PHE	LEU
6	GLY	ASP	ALA	VAL	ASP	LEU	THR	CYS	PHE	ASP
7	PHE	SER	ARG	ALA	GLY	SER	PRO	GLY	ASP	GLY

1	GLY	SER	LEU	ARG	VAL	LYS	GLY	LEU	PRO	SER
2	GLY	TRP	LYS	ARG	GLU	VAL	VAL	VAL	ARG	LYS
3	ASN	GLY	GLN	SER	ALA	GLY	LYS	THR	ASP	VAL
4	TYR	TYR	PHE	SER	PRO	CYS	GLY	LYS	LYS	PHE
5	ARG	SER	LYS	PRO	GLN	ILE	ALA	ARG	PHE	LEU
6	GLY	ASP	ALA	VAL	ASP	LEU	THR	CYS	PHE	ASP
7	PHE	SER	ARG	ALA	GLY	SER	PRO	GLY	ASP	GLY