BMRB Entry 26939

Name: Iminoproton chemical shifts of ASH1 E3 42mer RNA stem-loop
Published: 2016-12-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26939

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Iminoproton chemical shifts of ASH1 E3 42mer RNA stem-loop PubMed: 28092367

Deposition date: 2016-11-09 Original release date: 2016-12-29

Authors: Schlundt, Andreas; Sattler, Michael; Edelmann, Franziska; Niessing, Dierk

Citation: Edelmann, Franziska; Schlundt, Andreas; Heym, Roland; Jenner, Andreas; Niedner-Boblenz, Annika; Syed, Muhammad; Paillart, Jean-Christophe; Stehle, Ralf; Janowski, Robert; Sattler, Michael; Jansen, Ralf-Peter; Niessing, Dierk. "Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex" Nat. Struct. Biol. 24, 152-161 (2017).

Assembly members:
ASH1-E3_42mer, polymer, 42 residues, Formula weight is not available

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source: Production method: cell free synthesis Host organism: Saccharomyces cerevisiae Vector: PCR product

Entity Sequences (FASTA):
ASH1-E3_42mer: GAUAACUGAAUCGCUAAGGA UGAAAGUCUAUGCGACAUUA UC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	16

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ASH1-mRNA E3 42mer stem-loop	1

Entities:

Entity 1, ASH1-mRNA E3 42mer stem-loop 42 residues - Formula weight is not available

Sequence comprises three fragments corresponding to A, B and C in the CS files. A (1774-1785) and B (1803-1811) are strands of the wt stem sequence and C is an artificial stem-loop (gcuaaggaugaaagucuaugc).

1

G

A

U

A

C

U

G

A

2

U

C

G

C

U

A

G

A

3

U

G

A

G

U

C

U

A

4

U

G

C

G

A

C

A

U

A

5

U

C

Samples:

sample_1: ASH1-E3_42mer 200 uM; HEPES 20 mM; sodium chloride 150 mM; magnesium chloride 2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin, CCPN - chemical shift assignment, collection

NMR spectrometers:

Bruker Avance 900 MHz

Biological Magnetic Resonance Data Bank