BMRB Entry 26948

Name: Backbone chemical shift assignment of RNA recognition domain 2 of SART3
Published: 2018-03-26

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26948

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shift assignment of RNA recognition domain 2 of SART3

Deposition date:

2016-11-21

Original release date:

2018-03-26

Authors:

Kim, Nak-Kyoon; Kim, Won-Je; Bang, Kyeong-Mi; Shin, JiYeon

Citation:

Citation: Bang, Kyeong-Mi; Cho, Na Youn; Kim, Won-Je; Kim, Ae-Ryung; Song, Hyun Kyu; Kim, Eunice; Kim, Nak-Kyoon. "Structural Characterization of RNA Recognition Motif-2 Domain of SART3" Bull. Korean. Chem. Soc. 38, 444-447 (2017).

Assembly members:

Assembly members:
SART3_RRM2, polymer, 78 residues, 8682 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SART3_RRM2: KHKLFISGLPFSCTKEELEE ICKAHGTVKDLRLVTNRAGK PKGLAYVEYENESQASQAVM KMDGMTIKENIIKVAISN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	151
15N chemical shifts	74
1H chemical shifts	153

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SART3 RRM2	1

Entities:

Entity 1, SART3 RRM2 78 residues - 8682 Da.

78 residues

1

LYS

HIS

LYS

LEU

PHE

ILE

SER

GLY

LEU

PRO

2

PHE

SER

CYS

THR

LYS

GLU

LEU

GLU

3

ILE

CYS

LYS

ALA

HIS

GLY

THR

VAL

LYS

ASP

4

LEU

ARG

LEU

VAL

THR

ASN

ARG

ALA

GLY

LYS

5

PRO

LYS

GLY

LEU

ALA

TYR

VAL

GLU

TYR

GLU

6

ASN

GLU

SER

GLN

ALA

SER

GLN

ALA

VAL

MET

7

LYS

MET

ASP

GLY

MET

THR

ILE

LYS

GLU

ASN

8

ILE

LYS

VAL

ALA

ILE

SER

ASN

Samples:

sample_1: SART3 RRM2, [U-99% 13C; U-99% 15N], 1.2 mM; sodium phosphate 20 mM; NaCl 100 mM; DTT 0.5 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v3.114, Bruker Biospin, Goddard - chemical shift assignment, collection

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks