BMRB Entry 26953

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26953

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Kruppel-like Factor 4 (KLF4) Residues 1-130
Deposition date:
2016-11-22
Original release date:
2016-11-23
Authors:
Conroy, Brigid; Langelaan, David; Smith, Steven
Citation:

Citation: Conroy, Brigid; Weiss, Emma; Smith, Steven; Langelaan, David. "Backbone 1H, 13C, and 15N NMR resonance assignments of the Kruppel-like factor 4 activation domain"  Biomol. NMR Assign. 11, 95-98 (2017).
PubMed: 28247282

Assembly members:

Assembly members:
KLF4_1-130, polymer, 132 residues, 13757.2305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KLF4_1-130: GSMRQPPGESDMAVSDALLP SFSTFASGPAGREKTLRQAG APNNRWREELSHMKRLPPVL PGRPYDLAAATVATDLESGG AGAACGGSNLAPLPRRETEE FNDLLDLDFILSNSLTHPPE SVAATVSSSASA

Data sets:
Data typeCount
13C chemical shifts379
15N chemical shifts116
1H chemical shifts117

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1KLF4 1-1301

Entities:

Entity 1, KLF4 1-130 132 residues - 13757.2305 Da.

A Gly-Ser cloning artifact has been added to the N-terminus of the KLF4 1-130 fragment.

1   GLYSERMETARGGLNPROPROGLYGLUSER
2   ASPMETALAVALSERASPALALEULEUPRO
3   SERPHESERTHRPHEALASERGLYPROALA
4   GLYARGGLULYSTHRLEUARGGLNALAGLY
5   ALAPROASNASNARGTRPARGGLUGLULEU
6   SERHISMETLYSARGLEUPROPROVALLEU
7   PROGLYARGPROTYRASPLEUALAALAALA
8   THRVALALATHRASPLEUGLUSERGLYGLY
9   ALAGLYALAALACYSGLYGLYSERASNLEU
10   ALAPROLEUPROARGARGGLUTHRGLUGLU
11   PHEASNASPLEULEUASPLEUASPPHEILE
12   LEUSERASNSERLEUTHRHISPROPROGLU
13   SERVALALAALATHRVALSERSERSERALA
14   SERALA

Related Database Links:

UNP O43474
AlphaFold Q9UNP3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks