BMRB Entry 26966

Name: Backbone 1H, 13C, 15N backbone assignment of the human Heat Labile Enterotoxin (hLTB)
Published: 2017-03-31

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26966

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone 1H, 13C, 15N backbone assignment of the human Heat Labile Enterotoxin (hLTB) PubMed: 28243889

Deposition date: 2016-12-07 Original release date: 2017-03-31

Authors: Hatlem, Daniel; Heggelund, Julie; Burschowsky, Daniel; Krengel, Ute; Kristiansen, Per Eugen

Citation: Hatlem, Daniel; Heggelund, Julie; Burschowsky, Daniel; Krengel, Ute; Kristiansen, Per Eugen. "(1)H, (13)C, (15)N backbone assignment of the human heat-labile enterotoxin B-pentamer and chemical shift mapping of neolactotetraose binding" Biomol. NMR Assign. 11, 99-9104 (2017).

Assembly members:
human_Heat_Labile_Enterotoxin_B-subunit, polymer, 103 residues, 11702.38 Da.

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Vibrio sp. 60 Vector: pMMB66EH

Entity Sequences (FASTA):
human_Heat_Labile_Enterotoxin_B-subunit: APQSITELCSEYHNTQIYTI NDKILSYTESMAGKREMVII TFKSGATFQVEVPGSQHIDS QKKAIERMKDTLRITYLTET KIDKLCVWNNKTPNSIAAIS MEN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	256
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hLTB subunit, chain 1	1
2	hLTB subunit, chain 2	1
3	hLTB subunit, chain 3	1
4	hLTB subunit, chain 4	1
5	hLTB subunit, chain 5	1

Entities:

Entity 1, hLTB subunit, chain 1 103 residues - 11702.38 Da.

1

ALA

PRO

GLN

SER

ILE

THR

GLU

LEU

CYS

SER

2

GLU

TYR

HIS

ASN

THR

GLN

ILE

TYR

THR

ILE

3

ASN

ASP

LYS

ILE

LEU

SER

TYR

THR

GLU

SER

4

MET

ALA

GLY

LYS

ARG

GLU

MET

VAL

ILE

5

THR

PHE

LYS

SER

GLY

ALA

THR

PHE

GLN

VAL

6

GLU

VAL

PRO

GLY

SER

GLN

HIS

ILE

ASP

SER

7

GLN

LYS

ALA

ILE

GLU

ARG

MET

LYS

ASP

8

THR

LEU

ARG

ILE

THR

TYR

LEU

THR

GLU

THR

9

LYS

ILE

ASP

LYS

LEU

CYS

VAL

TRP

ASN

10

LYS

THR

PRO

ASN

SER

ILE

ALA

ILE

SER

11

MET

GLU

ASN

Samples:

sample_1: human Heat Labile Enterotoxin B-subunit, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mM; D2O, [U-99% 2H], 7%; sodium azide 0.02%; DSS 0.2 mM; sodium chloride 50 mM; potassium phosphate 15 mM

sample_conditions_1: ionic strength: 104 mM; pH: 6.5; pressure: 1 atm; temperature: 308.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY-HSQC	sample_1	isotropic	sample_conditions_1
3D TROSY-HNCO	sample_1	isotropic	sample_conditions_1
3D TROSY-HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D TROSY-HNCA	sample_1	isotropic	sample_conditions_1
3D TROSY-HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D TROSY-HNCACB	sample_1	isotropic	sample_conditions_1
3D TROSY-HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.6, Bruker Biospin - collection, processing

CARA v1.8.4.2, Rochus Keller - chemical shift assignment

NMR spectrometers:

Bruker Avance II 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts