BMRB Entry 26969
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26969
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone resonance assignments of the Pseudomonas aeruginosa major pilin PilA from strain PA14 PubMed: 29342354
Deposition date: 2016-12-07 Original release date: 2018-06-26
Authors: Nguyen, Ylan; McNicholl, Tyler; Akimoto, Madoka; Aidoo, Francisca; Melacini, Giuseppe; Burrows, Lori
Citation: Nguyen, Ylan; Boulton, Stephen; McNicholl, E Tyler; Akimoto, Madoka; Harvey, Hanjeong; Aidoo, Francisca; Melacini, Giuseppe; Burrows, Lori. "A Highly Dynamic Loop of the Pseudomonas aeruginosa PA14 Type IV Pilin Is Essential for Pilus Assembly." ACS Infect. Dis. 4, 936-943 (2018).
Assembly members:
PilA, polymer, 151 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET151
Entity Sequences (FASTA):
PilA: GIDPFTIRARVTEGVGLAAS
AKTLIGDSSATAGELAASAR
VWNAQAGNAGATSKYVTSVR
IAEATGEITVTFNATNVGNI
PADSTLVFTPYVQNAAGAPT
QLGASYAAGVTGSIDWGCAS
ESNAVSSGPDRNMPALTAGT
LPARFAPSECR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 434 |
| 15N chemical shifts | 139 |
| 1H chemical shifts | 139 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PilA | 1 |
Entities:
Entity 1, PilA 151 residues - Formula weight is not available
residues 1-6 are artifacts of affinity tag cleavage
| 1 | GLY | ILE | ASP | PRO | PHE | THR | ILE | ARG | ALA | ARG | ||||
| 2 | VAL | THR | GLU | GLY | VAL | GLY | LEU | ALA | ALA | SER | ||||
| 3 | ALA | LYS | THR | LEU | ILE | GLY | ASP | SER | SER | ALA | ||||
| 4 | THR | ALA | GLY | GLU | LEU | ALA | ALA | SER | ALA | ARG | ||||
| 5 | VAL | TRP | ASN | ALA | GLN | ALA | GLY | ASN | ALA | GLY | ||||
| 6 | ALA | THR | SER | LYS | TYR | VAL | THR | SER | VAL | ARG | ||||
| 7 | ILE | ALA | GLU | ALA | THR | GLY | GLU | ILE | THR | VAL | ||||
| 8 | THR | PHE | ASN | ALA | THR | ASN | VAL | GLY | ASN | ILE | ||||
| 9 | PRO | ALA | ASP | SER | THR | LEU | VAL | PHE | THR | PRO | ||||
| 10 | TYR | VAL | GLN | ASN | ALA | ALA | GLY | ALA | PRO | THR | ||||
| 11 | GLN | LEU | GLY | ALA | SER | TYR | ALA | ALA | GLY | VAL | ||||
| 12 | THR | GLY | SER | ILE | ASP | TRP | GLY | CYS | ALA | SER | ||||
| 13 | GLU | SER | ASN | ALA | VAL | SER | SER | GLY | PRO | ASP | ||||
| 14 | ARG | ASN | MET | PRO | ALA | LEU | THR | ALA | GLY | THR | ||||
| 15 | LEU | PRO | ALA | ARG | PHE | ALA | PRO | SER | GLU | CYS | ||||
| 16 | ARG |
Samples:
sample_1: PilA, [U-13C; U-15N], 0.5 mM; TRIS 20 mM; sodium chloride 50 mM
sample_2: PilA, [U-15N]-Leu, 0.5 mM; TRIS 20 mM; sodium chloride 50 mM
sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 306 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N NOESY-HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.113, Goddard - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 700 MHz
- Varian INOVA 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts