BMRB Entry 26982
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26982
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Title: Chemical shift assignments of the RYBP NZF domain PubMed: 29320735
Deposition date: 2016-12-21 Original release date: 2018-02-09
Authors: Lee, Brian; Spyracopoulos, Leo
Citation: Ali, Mohammad; Strickfaden, Hilmar; Lee, Brian; Spyracopoulos, Leo; Hendzel, Michael. "RYBP Is a K63-Ubiquitin-Chain-Binding Protein that Inhibits Homologous Recombination Repair" Cell Rep. 22, 383-395 (2018).
Assembly members:
RYBP_NZF_domain, polymer, 37 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P-1
Entity Sequences (FASTA):
RYBP_NZF_domain: GPLGSDEGFWDCSVCTFRNS
AEAFKCSICDVRKGTST
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 144 |
15N chemical shifts | 35 |
1H chemical shifts | 197 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RYBP NZF domain | 1 |
2 | ZINC ION | 2 |
Entities:
Entity 1, RYBP NZF domain 37 residues - Formula weight is not available
1 | GLY | PRO | LEU | GLY | SER | ASP | GLU | GLY | PHE | TRP | ||||
2 | ASP | CYS | SER | VAL | CYS | THR | PHE | ARG | ASN | SER | ||||
3 | ALA | GLU | ALA | PHE | LYS | CYS | SER | ILE | CYS | ASP | ||||
4 | VAL | ARG | LYS | GLY | THR | SER | THR |
Entity 2, ZINC ION - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: RYBP NZF domain, [U-13C; U-15N], 0.2 mM; DSS, [U-2H], 1 mM; sodium phosphate 20 mM; sodium chloride 150 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C TOCSY aliphatic | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
2D HBCBCGCDHD | sample_1 | isotropic | sample_conditions_1 |
2D HBCBCGCDCEHE | sample_1 | isotropic | sample_conditions_1 |
Software:
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts